Rabbit anti-Human CALU Polyclonal Antibody | anti-CALU antibody

CALU, CT (CALU, Calumenin, Crocalbin, IEF SSP 9302) (PE)

Cat. Num. AAA6280583

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CALU; Polyclonal Antibody; CT (CALU; Calumenin; Crocalbin; IEF SSP 9302) (PE); IEF SSP 9302; anti-CALU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CALU antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CALU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human CALU.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CALU Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CALU antibody detected the CALU protein (arrow).)

Immunohistochemistry (IHC)

(CALU Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CALU Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CALU antibody

The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-CALU antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Carlquist, J.F., et al. J. Thromb. Thrombolysis 30(3):358-364(2010). Hernandez-Romero, D., et al. Ann. Med. 42(6):439-446(2010). Harada, T., et al. Thromb. Res. 126(3):183-190(2010). Voora, D., et al. Clin. Pharmacol. Ther. 87(4):445-451(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 194578885
• NCBI GeneID: 813
• NCBI Accession #: NP_001124146.1; NP_001210.1
• NCBI GenBank Nucleotide #: NM_001130674.2
• UniProt Accession #: O43852; B3KPG9; D6QS48; D6QS49; D6QS50; D6QS51; D6QS52; D6QS53; D6QS54; D6QS55; D6QS56; D6QS57
• Molecular Weight: 19,455 Da
• NCBI Official Full Name: calumenin isoform b
• NCBI Official Synonym Full Names: calumenin
• NCBI Official Symbol: CALU
• NCBI Protein Information: calumenin; IEF SSP 9302; crocalbin-like protein; multiple EF-hand protein
• UniProt Protein Name: Calumenin
• Protein Family: Calumenin
• UniProt Gene Name: CALU
• UniProt Entry Name: CALU_HUMAN

NCBI Description

The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Uniprot Description

CALU: Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity. Belongs to the CREC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q32.1

Cellular Component: Golgi apparatus; sarcoplasmic reticulum lumen; membrane; endoplasmic reticulum; extracellular region; melanosome

Molecular Function: protein binding; calcium ion binding

Biological Process: platelet activation; platelet degranulation; blood coagulation