Testing Data
Sheep anti-Rat Calretinin Polyclonal Antibody | anti-CALB2 antibody
Anti-Calretinin
Reactivity
Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Protein A purified (Protein A purified sheep serum.)
Synonyms
Calretinin; Polyclonal Antibody; Anti-Calretinin; anti-CALB2 antibody
Host
Sheep
Reactivity
Rat
Clonality
Polyclonal
Specificity
Specific for the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for Immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
Purity/Purification
Protein A purified (Protein A purified sheep serum.)
Form/Format
100 ul in 10 mM Tris, 100 mM NaCl.
Sequence Length
271
Applicable Applications for anti-CALB2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
Quality Control: Western blots performed on each lot.
WB: 1:1,000
IHC: 1:500
IF: 1:500
WB: 1:1,000
IHC: 1:500
IF: 1:500
Antigen
Purified guinea pig calretinin.
Immunogen Information
Purified guinea pig calretinin
Immunogen Species
Guinea pig
Reactivity Assumed Based on 100% Sequence Homology
Human, mouse, non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in rat. It is expected that this antibody will also react with human, mouse and non-human primates.
Biological Significance
Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-CALB2 antibody
Sheep polyclonal antibody
References
• Attanoos RL, Dojcinov SD, Webb R, Gibbs AR. (2000) Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology.37(3):224-31.
• Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.
• Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.
• Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.
• Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.
• Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.
• Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29
NCBI Official Full Name
calretinin
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
Calb2
NCBI Protein Information
calretinin; CR
UniProt Protein Name
Calretinin
Protein Family
UniProt Gene Name
Calb2
UniProt Synonym Gene Names
CR
UniProt Entry Name
CALB2_RAT
NCBI Description
displays increased mRNA expression following trimethyltin-induced neurodegeneration [RGD, Feb 2006]
Uniprot Description
Function: Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Sequence similarities: Belongs to the calbindin family.Contains 6 EF-hand domains.
Research Articles on CALB2
Similar Products
Product Notes
The CALB2 calb2 (Catalog #AAA502148) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calretinin reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calretinin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). Quality Control: Western blots performed on each lot. WB: 1:1,000 IHC: 1:500 IF: 1:500. Researchers should empirically determine the suitability of the CALB2 calb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calretinin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
