Rabbit anti-Human Calpain 6/CAPN6 Polyclonal Antibody | anti-CAPN6 antibody

Anti-Calpain 6/CAPN6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103620

• Gene Names: CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Calpain 6/CAPN6; Polyclonal Antibody; Anti-Calpain 6/CAPN6 Antibody; Rabbit Polyclonal; Calpain 6/CAPN6 Antibody; Rabbit PAb; Antigen Affinity Purified; calpain 6; Anti-CalpM Antibody; Anti-CANPX Antibody; Anti-CAPNX Antibody; Anti-DJ914P14.1 Antibody; anti-CAPN6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Calpain 6/CAPN6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CAPN6 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human Calpain 6/CAPN6
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Calpain 6/CAPN6, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CAPN6 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CAPN6 antibody

Calpains are Ca(2+)-dependent modulator Cys proteases that have a variety of functions in almost all eukaryotes. There are more than 10 well-conserved mammalian calpains, among which eutherian calpain-6 (CAPN6) is unique in that it has amino acid substitutions at the active-site Cys residue (to Lys in humans), strongly suggesting a loss of proteolytic activity. CAPN6 is expressed predominantly in embryonic muscles, placenta, and several cultured cell lines. The inflammatory cytokines induce an unconventional nonproteolytic calpain, calpain-6 (CAPN6), which associates with the essential EJC-loading factor CWC22 in the cytoplasm. This association disturbs the nuclear localization of CWC22, thereby suppressing the splicing of target genes, including those related to Rac1 signaling. CAPN6 deficiency in LDL receptor-deficient mice restored CWC22/EJC/Rac1 signaling, reduced pinocytotic deposition of native LDL in macrophages, and attenuated macrophage recruitment into the lesions, generating an atheroprotective phenotype in the aorta. That CAPN6 promotes atherogenicity in inflamed macrophages by disturbing CWC22/EJC systems. Alpain 6 (CAPN6) expression is regulated by PI3K-Akt in liver cancer through POU2F1 and CAPN6 which promote cell proliferation and inhibit apoptosis of liver cancer cells. Loss of CAPN6 promotes skeletal muscle differentiation during both development and regeneration, suggesting a novel physiological function of CAPN6 as a suppressor of skeletal muscle differentiation. CAPN6 promoted cancer cell proliferation and inhibited its apoptosis. CAPN6 acts as a potential regulator of Rac1 activity, through a mechanism involving interaction with GEF-H1, to control lamellipodial formation and cell motility.

Product Categories/Family for anti-CAPN6 antibody

T Cell Receptor Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 13959315
• NCBI GeneID: 827
• UniProt Accession #: Q9Y6Q1; Q9UEQ1; Q9UJA8; D3DUY7
• Molecular Weight: 641
• NCBI Official Full Name: Calpain-6
• NCBI Official Synonym Full Names: calpain 6
• NCBI Official Symbol: CAPN6
• NCBI Official Synonym Symbols: CANPX; CAPNX; CalpM; DJ914P14.1
• NCBI Protein Information: calpain-6; calpamodulin; calpain-like protease X-linked
• UniProt Protein Name: Calpain-6
• Protein Family: Calpain
• UniProt Gene Name: CAPN6
• UniProt Synonym Gene Names: CALPM; CANPX; CalpM
• UniProt Entry Name: CAN6_HUMAN

NCBI Description

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]

Uniprot Description

CAPN6: Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues. Belongs to the peptidase C2 family.

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: spindle microtubule; perinuclear region of cytoplasm

Molecular Function: microtubule binding; calcium-dependent cysteine-type endopeptidase activity

Biological Process: proteolysis; microtubule bundle formation; regulation of cytoskeleton organization and biogenesis