Human Calpain 6 (CAPN6) RTU ELISA Kit | CAPN6 rtu elisa kit

Human Calpain 6 (CAPN6) ELISA Kit

Cat. Num. AAA458109

• Gene Names: CAPN6; CANPX; CAPNX; CalpM; DJ914P14.1
• Reactivity: Human
• Synonyms: Calpain 6 (CAPN6); Human Calpain 6 (CAPN6) ELISA Kit; CAPNX; CalpM; CANPX; Calpamodulin; Calpain-like protease X-linked; CAPN6 rtu elisa kit

• Reactivity: Human
• Sequence Length: 641

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAPN6 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAPN6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAPN6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAPN6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CAPN6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13186316
• NCBI GeneID: 827
• NCBI Accession #: NP_055104.2
• NCBI GenBank Nucleotide #: NM_014289.3
• UniProt Accession #: Q9Y6Q1; Q9UEQ1; Q9UJA8; D3DUY7
• Molecular Weight: 74,576 Da
• NCBI Official Full Name: calpain-6
• NCBI Official Synonym Full Names: calpain 6
• NCBI Official Symbol: CAPN6
• NCBI Official Synonym Symbols: CANPX; CAPNX; CalpM; DJ914P14.1
• NCBI Protein Information: calpain-6
• UniProt Protein Name: Calpain-6
• Protein Family: Calpain
• UniProt Gene Name: CAPN6
• UniProt Synonym Gene Names: CALPM; CANPX; CalpM

NCBI Description

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis. [provided by RefSeq, Nov 2009]

Uniprot Description

Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues ().