Rabbit anti-Human, Mouse Calnexin Polyclonal Antibody | anti-CANX antibody

Calnexin

Cat. Num. AAA622497

• Gene Names: CANX; CNX; P90; IP90; FLJ26570
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Calnexin; Polyclonal Antibody; Anti -Calnexin; anti-CANX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human calnexin protein. Species Crossreactivity: mouse
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.

• Applicable Applications for anti-CANX antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot and Flow Cytometry.; Dilution: ELISA: 0.05-0.2ug/ml; Western Blot: 0.5-1ug/ml; Flow Cytometry: 1-3ug to label 1x10e6 cells
• Immunogen: Synthetic peptide from carboxyl terminal of human calnexin protein, conjugated to KLH (Swiss Prot: P27824). Species Sequence Homology: rat, bovine, canine and Sumatran orangutan
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Ramos cells using MBS622497 (shaded histogram) or with I1904-30H (open histogram), followed by IgG (PE) anti-rabbit.)

Western Blot (WB)

(Western Blot analysis of cell lysates, using MBS622497 (1ug/ml).)

Related Product Information for anti-CANX antibody

Calnexin is calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may be involved in assisting protein assembly or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the ER quality control apparatus via the retention of incorrectly folded proteins.

Product Categories/Family for anti-CANX antibody

Antibodies; Abs to Calnexin

NCBI and Uniprot Product Information

• NCBI GI #: 186523
• NCBI GeneID: 821
• UniProt Accession #: P27824; B2R5V8; D3DWQ3; Q6ZP56
• Molecular Weight: 67,568 Da
• NCBI Official Full Name: calnexin
• NCBI Official Synonym Full Names: calnexin
• NCBI Official Symbol: CANX
• NCBI Official Synonym Symbols: CNX; P90; IP90; FLJ26570
• NCBI Protein Information: calnexin; OTTHUMP00000161516; OTTHUMP00000223184; OTTHUMP00000223187; major histocompatibility complex class I antigen-binding protein p88
• UniProt Protein Name: Calnexin
• Protein Family: Calnexin
• UniProt Gene Name: CANX
• UniProt Entry Name: CALX_HUMAN

NCBI Description

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Uniprot Description

Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum .Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

Protein type: Endoplasmic reticulum; Calcium-binding; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: dendrite cytoplasm; endoplasmic reticulum membrane; protein complex; smooth endoplasmic reticulum; rough endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; dendritic spine; membrane; cell soma; axon; melanosome; ribosome

Molecular Function: ionotropic glutamate receptor binding; protein binding; unfolded protein binding; apolipoprotein binding; calcium ion binding; carbohydrate binding; glycoprotein binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; synaptic vesicle endocytosis; protein secretion; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging