Rabbit anti-Human CALNA2 Polyclonal Antibody | anti-CALNA2 antibody

CALNA2, NT (Calmodulin-dependent Calcineurin A Subunit, beta Isoform, Serine/Threonine Protein Phosphatase 2B Catalytic Subunit, beta Isoform, CAM-PRP Catalytic Subunit, PPP3CB, CNA2) (MaxLight 650)

Cat. Num. AAA6466544

• Gene Names: PPP3CB; CNA2; CALNB; CALNA2; PP2Bbeta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CALNA2; Polyclonal Antibody; NT (Calmodulin-dependent Calcineurin A Subunit; beta Isoform; Serine/Threonine Protein Phosphatase 2B Catalytic Subunit; CAM-PRP Catalytic Subunit; PPP3CB; CNA2) (MaxLight 650); anti-CALNA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPP3CB.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-CALNA2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PPP3CB (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PPP3CB Pab in mouse kidney tissue lysate. PPP3CB(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-PPP3CB Antibody (N-term) in Jurkat cell line lysates (35ug/lane). PPP3CB(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CALNA2 antibody

Antibodies; Enzymes; Phosphatase

References

1. Katanosaka, Y., et al., J. Biol. Chem. 280(7):5764-5772 (2005). 2. Bell, O., et al., Mol. Endocrinol. 19(2):516-526 (2005). 3. Bennasser, Y., et al., Virology 303(1):174-180 (2002). 4. Kissinger, C.R., et al., Nature 378(6557):641-644 (1995). 5. Muramatsu, T., et al., Biochim. Biophys. Acta 1178(1):117-120 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 11036640
• NCBI GeneID: 5532
• NCBI Accession #: NP_066955
• NCBI GenBank Nucleotide #: NM_021132.3
• UniProt Accession #: P16298; P16299; Q5F2F9; Q8N1F0; Q8N3W4
• Molecular Weight: 59,123 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2B catalytic subunit beta isoform isoform b
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, beta isozyme
• NCBI Official Symbol: PPP3CB
• NCBI Official Synonym Symbols: CNA2; CALNB; CALNA2; PP2Bbeta
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
• UniProt Gene Name: PPP3CB
• UniProt Synonym Gene Names: CALNA2; CALNB; CNA2
• UniProt Entry Name: PP2BB_HUMAN

Uniprot Description

PPP3CB: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A beta. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: nucleoplasm; T-tubule; plasma membrane; Z disc; cytosol; calcineurin complex

Molecular Function: protein dimerization activity; calmodulin binding; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; calcium ion binding; protein serine/threonine phosphatase activity; drug binding; protein phosphatase 2B binding

Biological Process: T cell activation; positive regulation of NFAT protein import into nucleus; axon extension; heart development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; learning; social behavior; signal transduction; protein amino acid dephosphorylation; protein amino acid phosphorylation; memory; calcium ion-dependent exocytosis; locomotion during locomotory behavior; T cell proliferation; calcineurin-NFAT signaling pathway; dephosphorylation; response to cytokine stimulus; negative regulation of T cell mediated cytotoxicity; innate immune response; T cell homeostasis; positive regulation of transcription from RNA polymerase II promoter; regulation of insulin secretion; T cell differentiation