Rabbit anti-Human, Mouse CALCOCO1 Polyclonal Antibody | anti-CALCOCO1 antibody

CALCOCO1 Antibody

Cat. Num. AAA7045520

• Gene Names: CALCOCO1; Cocoa; PP13275; calphoglin
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: CALCOCO1; Polyclonal Antibody; CALCOCO1 Antibody; Calcium-binding and coiled-coil domain-containing protein 1; Calphoglin; Coiled-coil coactivator protein; Sarcoma antigen NY-SAR-3; KIAA1536; PP13275; UNQ2436/PRO4996; anti-CALCOCO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 606

• Applicable Applications for anti-CALCOCO1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Calcium-binding and coiled-coil domain-containing protein 1 protein (C-230AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-161423 / sc-161424
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes:  CALCOCO1antibody at 1.61ug/mlLane 1 : 293T whole cell lysateLane 2 : MCF7 whole cell lysateLane 3 : Mouse lung tissueSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 78,71,68 kDaObserved band size: 77 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7045520 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7045520 at dilution 1:100)

Related Product Information for anti-CALCOCO1 antibody

Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity). In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

NCBI and Uniprot Product Information

• NCBI GI #: 219521893
• NCBI GeneID: 57658
• NCBI Accession #: NP_001137154.1
• NCBI GenBank Nucleotide #: NM_001143682.1
• UniProt Accession #: Q9P1Z2; Q6FI59; Q71RC3; Q86WF8; Q96JU3; Q9H090; B3KVA8
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: calcium-binding and coiled-coil domain-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: calcium binding and coiled-coil domain 1
• NCBI Official Symbol: CALCOCO1
• NCBI Official Synonym Symbols: Cocoa; PP13275; calphoglin
• NCBI Protein Information: calcium-binding and coiled-coil domain-containing protein 1
• UniProt Protein Name: Calcium-binding and coiled-coil domain-containing protein 1
• Protein Family: Calcium-binding and coiled-coil domain-containing protein
• UniProt Gene Name: CALCOCO1
• UniProt Synonym Gene Names: KIAA1536
• UniProt Entry Name: CACO1_HUMAN

Uniprot Description

CALCOCO1: Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1- mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions. Belongs to the CALCOCO family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: beta-catenin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; sequence-specific DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: positive regulation of transcription, DNA-dependent; signal transduction; steroid hormone receptor signaling pathway; transcription, DNA-dependent