Rabbit Calcineurin A Polyclonal Antibody | anti-PPP3CB antibody

Calcineurin A Antibody

Cat. Num. AAA840396

• Gene Names: PPP3CB; CNA2; CALNB; CALNA2; PP2Bbeta
• Reactivity: Human, Mouse, Rat, Hamster, Rabbit, Bovine
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: Calcineurin A; Polyclonal Antibody; Calcineurin A Antibody; PPP3CA; CCN1; CNA1; CALN; CALNA; CALNA1; PPP2B; anti-PPP3CB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Hamster, Rabbit, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) affinity purified, rabbit anti-Calcineurin A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 525

• Applicable Applications for anti-PPP3CB antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western Blotting (1-4 ug/ml) and for immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A..
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 267 of human Calcineurin A
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-PPP3CB antibody

Background: Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drmugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 215272352
• NCBI GeneID: 5532
• NCBI Accession #: NP_001135825.1
• NCBI GenBank Nucleotide #: NM_001142353.2
• UniProt Accession #: P16298; P16299; Q5F2F9; Q8N1F0; Q8N3W4
• Molecular Weight: 59,123 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2B catalytic subunit beta isoform isoform a
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, beta isozyme
• NCBI Official Symbol: PPP3CB
• NCBI Official Synonym Symbols: CNA2; CALNB; CALNA2; PP2Bbeta
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP3CB
• UniProt Synonym Gene Names: CALNA2; CALNB; CNA2
• UniProt Entry Name: PP2BB_HUMAN

Uniprot Description

PPP3CB: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A beta. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: nucleoplasm; T-tubule; plasma membrane; cytosol; Z disc; calcineurin complex

Molecular Function: protein dimerization activity; calmodulin binding; protein binding; calmodulin-dependent protein phosphatase activity; enzyme binding; protein serine/threonine phosphatase activity; calcium ion binding; drug binding; protein phosphatase 2B binding

Biological Process: T cell activation; positive regulation of NFAT protein import into nucleus; axon extension; heart development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; social behavior; learning; signal transduction; protein amino acid dephosphorylation; protein amino acid phosphorylation; memory; calcium ion-dependent exocytosis; locomotion during locomotory behavior; T cell proliferation; calcineurin-NFAT signaling pathway; dephosphorylation; response to cytokine stimulus; negative regulation of T cell mediated cytotoxicity; innate immune response; T cell homeostasis; positive regulation of transcription from RNA polymerase II promoter; regulation of insulin secretion; T cell differentiation