Western Blot (WB)
(Anti- Calcineurin A Picoband antibody, MBS177732, Western blottingAll lanes: Anti Calcineurin A (MBS177732) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 59KDObserved bind size: 84KD)
anti-Human Calcineurin A Polyclonal Antibody | anti-PPP3CA antibody
Anti-Calcineurin A Antibody
Western Blot (WB)
(Anti- Calcineurin A Picoband antibody, MBS177732, Western blottingAll lanes: Anti Calcineurin A (MBS177732) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 59KDObserved bind size: 84KD)
Western Blot (WB)
(Anti- Calcineurin A Picoband antibody, MBS177732, Western blottingAll lanes: Anti Calcineurin A (MBS177732) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 59KDObserved bind size: 84KD)
Background: Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.
NCBI and Uniprot Product Information
Uniprot Description
PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: calcineurin complex; cytoplasm; cytosol; mitochondrion; nucleoplasm; sarcolemma; Z disc
Molecular Function: calcium ion binding; calmodulin binding; calmodulin-dependent protein phosphatase activity; drug binding; enzyme binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein serine/threonine phosphatase activity
Biological Process: calcineurin-NFAT signaling pathway; calcium ion transport; cardiac muscle hypertrophy; dephosphorylation; G1/S transition of mitotic cell cycle; multicellular organismal response to stress; negative regulation of dendrite morphogenesis; negative regulation of insulin secretion; positive regulation of NFAT protein import into nucleus; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; protein import into nucleus; regulation of excitatory postsynaptic membrane potential; regulation of synaptic transmission; response to amphetamine; response to calcium ion; skeletal muscle fiber development; T cell activation; transition between fast and slow fiber; Wnt receptor signaling pathway, calcium modulating pathway
Research Articles on PPP3CA
Similar Products
Product Notes
The PPP3CA ppp3ca (Catalog #AAA177732) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Calcineurin A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calcineurin A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the PPP3CA ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcineurin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
