Rabbit anti-Mouse Cadherin, Neuronal (CDH2) Polyclonal Antibody | anti-CDH2 antibody

Polyclonal Antibody to Cadherin, Neuronal (CDH2)

Cat. Num. AAA2006280

• Gene Names: Cdh2; CDHN; Ncad; N-CAD
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Cadherin; Neuronal (CDH2); Polyclonal Antibody; Polyclonal Antibody to Cadherin; anti-CDH2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against CDH2. It has been selected for its ability to recognize CDH2 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-LPKDVH EGQPLLNVKF SNCNRKRKVQ YESSEPADFK VDEDGTVYAV RSFPLTAEQA KFLIYAQDKE TQEKWQVAVN LSREPTLTEE PMKEPHEIEE IVFPRQLAKH SGALQRQKRD WVIPPINLPE NSRGPFPQEL VRIRSDRDKN LSLRYSVTGP GADQPPTGIF IINPISGQLS VTKPLDRELI ARFHLRAH

• Applicable Applications for anti-CDH2 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant CDH2 (Leu45~His238) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055721
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Hela Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant CDH2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Testis Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Mouse Heart Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 161760627
• NCBI GeneID: 12558
• NCBI Accession #: NP_031690.3
• NCBI GenBank Nucleotide #: NM_007664.5
• UniProt Accession #: P15116; Q64260; Q6GU11
• Molecular Weight: 99,796 Da
• NCBI Official Full Name: cadherin-2 preproprotein
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: Cdh2
• NCBI Official Synonym Symbols: CDHN; Ncad; N-CAD
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• Protein Family: Cadherin
• UniProt Gene Name: Cdh2
• UniProt Synonym Gene Names: N-cadherin

NCBI Description

This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Mice lacking the encoded protein exhibit severe developmental defects resulting in embryonic death. [provided by RefSeq, Oct 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.