Western Blot (WB)
(Western blot analysis of CABYR using NIH-3T3 whole cell lysates)
Rabbit anti-Human CABYR Polyclonal Antibody | anti-CABYR antibody
CABYR Antibody
Gene Names
CABYR; CT88; FSP2; CBP86; FSP-2; CABYRa; CABYRc; CABYRe; CABYRc/d
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CABYR; Polyclonal Antibody; CABYR Antibody; CABYR_HUMAN; Calcium binding tyrosine-(Y)-phosphorylation regulated; Calcium-binding protein 86; Calcium-binding tyrosine phosphorylation-regulated protein; Cancer/testis antigen 88; CBP86; CT88; Fibrousheathin II; Fibrousheathin-2; FSP-2; FSP2; Testis-specific calcium-binding protein CBP86; anti-CABYR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CABYR antibody detects endogenous levels of total CABYR
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
493
Applicable Applications for anti-CABYR antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CABYR
Subcellular Location
Cytoplasm > Cytoskeleton. Cell projection > Cilium > Flagellum. Localized to fibrous sheath including the surface of the longitudinal columns and ribs of the principal piece of sperm flagella and nucleus. cytoplasm. Cell Projection > Cilium > Flagellum. According to PubMed:15752768, isoform 1, isoform 3 and isoform 5 are both nuclear and cytoplasmic.
Tissue Specificity
Expressed in elongating spermatids and spermatozoa (at protein level). Isoform 1 is expressed in testis. Isoform 3 and isoform 5 are also expressed in brain, pancreas and numerous brain tumors.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CABYR using NIH-3T3 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CABYR using NIH-3T3 whole cell lysates)
Related Product Information for anti-CABYR antibody
Function: May function as a regulator of both motility-and head-associated functions such as capacitation and the acrosome reaction. Isoform 1 binds calcium in vitro. Isoform 2 and isoform 6 probably bind calcium. Isoform 3 and isoform 5 do not bind calcium in vitro. Isoform 4 probably does not bind calcium.
Subunit Structure: Interacts with FSCB (By similarity). Isoform 3 self-associates. Isoform 3 and isoform 5 interact with GSK3B. Isoform 1 does not interact with GSK3B.
Post-translational Modifications: Isoform 1 is phosphorylated on tyrosine residues during in vitro capacitation. Isoform 3 and isoform 5 are phosphorylated by GSK3B in vitro. Dephosphorylation affects its ability to bind calcium.
Subunit Structure: Interacts with FSCB (By similarity). Isoform 3 self-associates. Isoform 3 and isoform 5 interact with GSK3B. Isoform 1 does not interact with GSK3B.
Post-translational Modifications: Isoform 1 is phosphorylated on tyrosine residues during in vitro capacitation. Isoform 3 and isoform 5 are phosphorylated by GSK3B in vitro. Dephosphorylation affects its ability to bind calcium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 53 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
calcium-binding tyrosine phosphorylation-regulated protein isoform a
NCBI Official Synonym Full Names
calcium binding tyrosine phosphorylation regulated
NCBI Official Symbol
CABYR
NCBI Official Synonym Symbols
CT88; FSP2; CBP86; FSP-2; CABYRa; CABYRc; CABYRe; CABYRc/d
NCBI Protein Information
calcium-binding tyrosine phosphorylation-regulated protein
UniProt Protein Name
Calcium-binding tyrosine phosphorylation-regulated protein
Protein Family
UniProt Gene Name
CABYR
UniProt Synonym Gene Names
CBP86; FSP2; CT88; FSP-2
NCBI Description
To reach fertilization competence, spermatozoa undergo a series of morphological and molecular maturational processes, termed capacitation, involving protein tyrosine phosphorylation and increased intracellular calcium. The protein encoded by this gene localizes to the principal piece of the sperm flagellum in association with the fibrous sheath and exhibits calcium-binding when phosphorylated during capacitation. A pseudogene on chromosome 3 has been identified for this gene. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
Uniprot Description
May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. Isoform 1 binds calcium in vitro. Isoform 2 and isoform 6 probably bind calcium. Isoform 3 and isoform 5 do not bind calcium in vitro. Isoform 4 probably does not bind calcium.
Research Articles on CABYR
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Product Notes
The CABYR cabyr (Catalog #AAA9609627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CABYR Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CABYR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CABYR cabyr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CABYR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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