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Western Blot (WB) (Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.)

Rabbit anti-Human CA14 Polyclonal Antibody | anti-CA14 antibody

CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV) (HRP)

Gene Names
CA14; CAXiV
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
CA14; Polyclonal Antibody; NT (CA14; Carbonic anhydrase 14; Carbonate dehydratase XIV; Carbonic anhydrase XIV) (HRP); Carbonic anhydrase XIV; anti-CA14 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-CA14 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
CA14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids from the N-terminal region of human CA14.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of CA14 antibody (N-term) in A375 cell line lysates (35ug/lane). CA14 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of A375 cells using CA14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.)

Western Blot (WB) (Western blot analysis of CA14 (arrow) using rabbit polyclonal CA14 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA14 gene.)
Product Categories/Family for anti-CA14 antibody
References
Parkkila, S. Natl. Acad. Sci. U.S.A. 98 (4), 1918-1923 (2001). Fujikawa-Adachi, K., Nishimori, I. Genomics 61 (1), 74-81 (1999). Tarun, A.S., Bryant, B. Chem. Senses 28 (7), 621-629 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,668 Da
NCBI Official Full Name
carbonic anhydrase 14
NCBI Official Synonym Full Names
carbonic anhydrase XIV
NCBI Official Symbol
CA14
NCBI Official Synonym Symbols
CAXiV
NCBI Protein Information
carbonic anhydrase 14; CA-XIV; carbonic dehydratase; carbonate dehydratase XIV
UniProt Protein Name
Carbonic anhydrase 14
Protein Family
UniProt Gene Name
CA14
UniProt Synonym Gene Names
CA-XIV
UniProt Entry Name
CAH14_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; EC 4.2.1.1; Membrane protein, integral; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; metal ion binding

Biological Process: bicarbonate transport

Research Articles on CA14

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Product Notes

The CA14 ca14 (Catalog #AAA6280203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CA14, NT (CA14, Carbonic anhydrase 14, Carbonate dehydratase XIV, Carbonic anhydrase XIV) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CA14 ca14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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