Typical Testing Data/Standard Curve (for reference only)
Mouse Periostin (POSTN) ELISA Kit | POSTN elisa kit
Mouse Periostin (POSTN) ELISA Kit
Gene Names
POSTN; PN; OSF2; OSF-2; PDLPOSTN
Reactivity
Mouse
Synonyms
Periostin (POSTN); Mouse Periostin (POSTN) ELISA Kit; OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2; POSTN elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of PER1. No significant cross-reactivity or interference between PER1 and analogues was observed.
Sequence Length
721
Samples
Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1,000pg/mL
Sensitivity
5.5pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PER1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PER1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for POSTN elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PER1 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PER1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PER1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PER1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PER1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PER1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PER1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,357 Da
NCBI Official Full Name
periostin isoform 1
NCBI Official Synonym Full Names
periostin
NCBI Official Symbol
POSTN
NCBI Official Synonym Symbols
PN; OSF2; OSF-2; PDLPOSTN
NCBI Protein Information
periostin
UniProt Protein Name
Periostin
Protein Family
UniProt Gene Name
POSTN
UniProt Synonym Gene Names
OSF2; PN; OSF-2
NCBI Description
This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Uniprot Description
Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX ().
Research Articles on POSTN
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Product Notes
The Mouse POSTN postn (Catalog #AAA2706381) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706381 ELISA Kit recognizes Mouse POSTN. It is sometimes possible for the material contained within the vial of "Periostin (POSTN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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