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Western Blot (WB) (Western blot analysis of C3orf75 Antibody (C-term) in K562 cell line lysates (35ug/lane).C3orf75 (arrow) was detected using the purified Pab.)

Rabbit anti-Human C3orf75 Polyclonal Antibody | anti-C3orf75 antibody

C3orf75, CT (C3orf75, ATP1, TMEM103, Elongator complex protein 6 homolog, Angiotonin-transactivated protein 1, Protein TMEM103) (Azide free) (HRP)

Gene Names
ELP6; C3orf75; TMEM103
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
C3orf75; Polyclonal Antibody; CT (C3orf75; ATP1; TMEM103; Elongator complex protein 6 homolog; Angiotonin-transactivated protein 1; Protein TMEM103) (Azide free) (HRP); Protein TMEM103; anti-C3orf75 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-C3orf75 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
C3orf75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human C3orf75.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of C3orf75 Antibody (C-term) in K562 cell line lysates (35ug/lane).C3orf75 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of C3orf75 Antibody (C-term) in K562 cell line lysates (35ug/lane).C3orf75 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(C3orf75 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C3orf75 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (C3orf75 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C3orf75 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(C3orf75 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (C3orf75 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-C3orf75 antibody
The function of this protein has not been specifically defined.
Product Categories/Family for anti-C3orf75 antibody
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
29,793 Da
NCBI Official Full Name
Chromosome 3 open reading frame 75
NCBI Official Synonym Full Names
elongator acetyltransferase complex subunit 6
NCBI Official Symbol
ELP6
NCBI Official Synonym Symbols
C3orf75; TMEM103
NCBI Protein Information
elongator complex protein 6; UPF0405 protein C3orf75; transmembrane protein 103; angiotonin-transactivated protein 1; elongator complex protein 6 homolog
UniProt Protein Name
Elongator complex protein 6
UniProt Gene Name
ELP6
UniProt Synonym Gene Names
ATP1; C3orf75; TMEM103
UniProt Entry Name
ELP6_HUMAN

Uniprot Description

Function: Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in cell migration.

Subunit structure: Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, ELP5 and ELP6. Ref.4

Sequence similarities: Belongs to the ELP6 family.

Sequence caution: The sequence AAH00623.1 differs from that shown. Reason: Frameshift at position 256. The sequence AAH14204.1 differs from that shown. Reason: Frameshift at position 256.

Research Articles on C3orf75

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Product Notes

The C3orf75 elp6 (Catalog #AAA6279862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C3orf75, CT (C3orf75, ATP1, TMEM103, Elongator complex protein 6 homolog, Angiotonin-transactivated protein 1, Protein TMEM103) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C3orf75 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the C3orf75 elp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3orf75, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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