SDS-Page
(SDS-PAGE Analysis Purified CD3e Hamster Monoclonal Antibody (145-2C11).)
CD3e Monoclonal Antibody | anti-Cd3e antibody
CD3e, Mouse
Gene Names
Cd3e; CD3; T3e; AI504783; CD3epsilon
Reactivity
MouseDoes not react with Human.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CD3e; Monoclonal Antibody; Mouse; CD3 epsilon; CD3e antigen epsilon polypeptide (TiT3 complex); CD3e molecule; epsilon (CD3-TCR complex); IMD18; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; anti-Cd3e antibody
Host
Armenian Hamster
Reactivity
Mouse
Does not react with Human.
Does not react with Human.
Clonality
Monoclonal
Isotype
IgG, kappa
Clone Number
145-2C11
Specificity
CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful forin vitroblocking and activation assays, as well as apoptosis induction andin vivoT cell depletion.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
189
Applicable Applications for anti-Cd3e antibody
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Functional Studies: Order Antibody without BSA & Azide
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 0.5-1ug/ml
FC/FACS: 0.5-1ug/million cells in 0.1ml
IF: 0.5-1ug/ml
Positive Control
MPBMC's or Thymus or Lymph Node.
Immunogen
H2Kb specific Mouse cytotoxic T lymphocyte clone BM10 37
Cellular Localization
Cell Surface
Hu-chromosome Location
9 A5.2; 9 24.84 cM (Mouse)
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified CD3e Hamster Monoclonal Antibody (145-2C11).)
SDS-Page
(SDS-PAGE Analysis Purified CD3e Hamster Monoclonal Antibody (145-2C11).)
References
1. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC). 2. Payer E, et al. 1991. J. Immunol. 146:2536. (IF). 3. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block). 4. Wang W, et al. 2007. J. Immunol. 178:4885. (Activation). 5. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete). 6. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB). 7. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activation)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23kDa
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3 antigen, epsilon polypeptide
NCBI Official Symbol
Cd3e
NCBI Official Synonym Symbols
CD3; T3e; AI504783; CD3epsilon
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
Protein Family
UniProt Gene Name
Cd3e
UniProt Entry Name
CD3E_MOUSE
Research Articles on Cd3e
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Product Notes
The Cd3e cd3e (Catalog #AAA4381454) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The CD3e, Mouse reacts with Mouse Does not react with Human. and may cross-react with other species as described in the data sheet. AAA Biotech's CD3e can be used in a range of immunoassay formats including, but not limited to, Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Functional Studies: Order Antibody without BSA & Azide FC/FACS: 0.5-1ug/million cells in 0.1ml IF: 0.5-1ug/ml. Researchers should empirically determine the suitability of the Cd3e cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3e, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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