Rabbit anti-Human C1QC Polyclonal Antibody | anti-C1QC antibody

C1QC, ID (C1QC, C1QG, Complement C1q subcomponent subunit C) (APC)

Cat. Num. AAA6279738

• Gene Names: C1QC; C1QG; C1Q-C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: C1QC; Polyclonal Antibody; ID (C1QC; C1QG; Complement C1q subcomponent subunit C) (APC); Complement C1q subcomponent subunit C; anti-C1QC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human C1QC.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-C1QC antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 93-120 in the central region of human C1QC, UniProt Accession #P02747.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS649251.This demonstrates that MBS649251 detected the C1QC protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue using MBS649251 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS649251 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HL-60 cells using MBS649251 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with WiDr cells using MBS649251 followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-C1QC antibody

Antibodies; Complement

References

1. Fraser, D.A., et al. J. Immunol. 185(7):3932-3939(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Rafiq, S., et al. Clin. Exp. Immunol. 161(2):284-289(2010). 4. Han, S., et al. Hum. Immunol. 71(7):727-730(2010). 5. Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 56786155
• NCBI GeneID: 714
• NCBI Accession #: NP_758957.2
• NCBI GenBank Nucleotide #: NM_172369.3
• UniProt Accession #: P02747; Q7Z502; Q96DL2; Q96H05
• Molecular Weight: 25,774 Da
• NCBI Official Full Name: complement C1q subcomponent subunit C
• NCBI Official Synonym Full Names: complement component 1, q subcomponent, C chain
• NCBI Official Symbol: C1QC
• NCBI Official Synonym Symbols: C1QG; C1Q-C
• NCBI Protein Information: complement C1q subcomponent subunit C; complement component 1, q subcomponent, gamma polypeptide
• UniProt Protein Name: Complement C1q subcomponent subunit C
• Protein Family: Complement C1q subcomponent
• UniProt Gene Name: C1QC
• UniProt Synonym Gene Names: C1QG
• UniProt Entry Name: C1QC_HUMAN

NCBI Description

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. A deficiency in C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N-terminus, and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the C-chain polypeptide of human complement subcomponent C1q. Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

C1QC: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. Defects in C1QC are a cause of complement component C1q deficiency (C1QD). A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; collagen; extracellular region

Biological Process: negative regulation of macrophage differentiation; innate immune response; immune response; complement activation, classical pathway; negative regulation of granulocyte differentiation; complement activation

Disease: C1q Deficiency