Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat C14orf68 Polyclonal Antibody | anti-C14orf68 antibody
Goat anti-C14orf68 Antibody
Gene Names
SLC25A47; HDMCP; C14orf68; HMFN1655
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
C14orf68; Polyclonal Antibody; Goat anti-C14orf68 Antibody; Goat Anti-C14orf68 (aa242-254) Antibody; chromosome 14 open reading frame 68; HDMCP; HMFN1655; hepatocellular carcinoma-downregulated mitochondrial carrier protein; C14orf68 antibody; chromosome 14 open reading frame 68 antibody; HDMCP antibody; HMFN1655 antibody; hepatocellular carcinoma-downregulated mitochondrial carrier protein antibody; anti-C14orf68 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RYGNPDAKPTKAD
Sequence Length
308
Applicable Applications for anti-C14orf68 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-RYGNPDAKPTKAD, from the internal region of the protein sequence according to NP_997000.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
2,904 Da
NCBI Official Full Name
solute carrier family 25 member 47
NCBI Official Synonym Full Names
solute carrier family 25 member 47
NCBI Official Symbol
SLC25A47
NCBI Official Synonym Symbols
HDMCP; C14orf68; HMFN1655
NCBI Protein Information
solute carrier family 25 member 47
UniProt Protein Name
Solute carrier family 25 member 47
UniProt Gene Name
SLC25A47
UniProt Synonym Gene Names
C14orf68; HDMCP
UniProt Entry Name
S2547_HUMAN
Uniprot Description
HDMCP: Uncoupling protein which may catalyze the physiological 'proton leak' in liver. Overexpression induces the dissipation of mitochondrial membrane potential. Belongs to the mitochondrial carrier family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial
Chromosomal Location of Human Ortholog: 14q32.2
Cellular Component: integral to membrane; mitochondrial inner membrane
Molecular Function: structural constituent of ribosome
Biological Process: translation; transmembrane transport
Research Articles on C14orf68
Similar Products
Product Notes
The C14orf68 slc25a47 (Catalog #AAA420743) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-C14orf68 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's C14orf68 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Liver shows staining of nucleoli in select hepatocytes. Recommended concentration, 2.5ug/ml. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4kDa according to NP_997000.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the C14orf68 slc25a47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RYGNPDAKPT KAD. It is sometimes possible for the material contained within the vial of "C14orf68, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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