Rabbit anti-Human, Mouse C14orf49 Polyclonal Antibody | anti-C14orf49 antibody

C14orf49 Antibody

Cat. Num. AAA9605677

• Gene Names: SYNE3; KASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: C14orf49; Polyclonal Antibody; C14orf49 Antibody; Chromosome 14 open reading frame 49; FLJ25605; MGC75267; Nesp3; Nesprin 3; Nesprin-3; NET53; Nuclear Envelope Spectrin Repeat Protein 3; Spectrin Repeat Containing; Nuclear Envelope Family Member 3; SYNE3; SYNE3_HUMAN; anti-C14orf49 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: C14orf49 antibody detects endogenous levels of total C14orf49
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 975

• Applicable Applications for anti-C14orf49 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF: 1:200
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus Outer Membrane. Located at the rough reticulum endoplasmic (RER) in some cells.
• Tissue Specificity: Expressed in aortic endothelial cells (at protein level).
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (100%), Bovine (91%), Horse (100%), Sheep (82%), Rabbit (82%), Dog (100%), Chicken (91%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells, using C14orf49 antibody.)

Immunofluorescene (IF)

(MBS9605677 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-C14orf49 antibody

Function: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.
Subunit Structure: Core component of LINC complexes which are composed of inner nuclear membrane SUN domain-containing proteins coupled to outer nuclear membrane KASH domain-containing nesprins. SUN and KASH domain-containing proteins seem to bind each other promiscuously; however, differentially expression of LINC complex constituents can give rise to specific assemblies. Interacts with SUN1 and SUN2; probably forming respective LINC complexes. Interacts with PLEC (via actin-binding domain). Interacts with DST. Interacts with SYNE1 via spectrin repeats. Interacts (via KASH domain) with TOR1A (ATP-bound); the interaction is required for SYNE3 nuclear envelope localization.
Post-translational Modifications: The disulfid bond with SUN1 or SUN2 is required for stability of the respective LINC complex under tensile forces.
Similarity: The KASH domain is involved in the binding to SUN1 and SUN2 through recognition of their SUN domains. Belongs to the nesprin family.

NCBI and Uniprot Product Information

• NCBI GI #: 145580592
• NCBI GeneID: 161176
• NCBI Accession #: NP_689805.3
• NCBI GenBank Nucleotide #: NM_152592.5
• UniProt Accession #: Q6ZMZ3; Q86SX5; Q8N7G8; A6H8H3
• Molecular Weight: Observed: 130 kD; Predicted: 113 kDa
• NCBI Official Full Name: nesprin-3 isoform 1
• NCBI Official Synonym Full Names: spectrin repeat containing nuclear envelope family member 3
• NCBI Official Symbol: SYNE3
• NCBI Official Synonym Symbols: KASH3; NET53; Nesp3; C14orf49; C14orf139; LINC00341; NCRNA00341
• NCBI Protein Information: nesprin-3
• UniProt Protein Name: Nesprin-3
• UniProt Gene Name: SYNE3
• UniProt Synonym Gene Names: KASH3

Uniprot Description

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.