Rabbit anti-Human, Mouse C14orf179 Polyclonal Antibody | anti-C14orf179 antibody

C14orf179, ID (IFT43, C14orf179, Intraflagellar transport protein 43 homolog) (FITC)

Cat. Num. AAA6279564

• Gene Names: IFT43; CED3; C14orf179
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C14orf179; Polyclonal Antibody; ID (IFT43; Intraflagellar transport protein 43 homolog) (FITC); IFT43; Intraflagellar transport protein 43 homolog; anti-C14orf179 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-C14orf179 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: C14orf179 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human C14orf179.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(C14orf179 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the C14orf179 antibody detected the C14orf179 protein (arrow).)

Immunohistochemistry (IHC)

(C14orf179 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C14orf179 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(C14orf179 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-C14orf179 antibody

Antibodies; Transport Proteins

References

Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007): Heilig, R., et al. Nature 421(6923):601-607(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 156523264
• NCBI GeneID: 112752
• NCBI Accession #: NP_001096034.1; NP_443105.2
• NCBI GenBank Nucleotide #: NM_001102564.1
• UniProt Accession #: Q96FT9; O95418; Q9ULA9; B3KPT6; B4DZI9; G3V385
• Molecular Weight: 23,529 Da
• NCBI Official Full Name: intraflagellar transport protein 43 homolog isoform 2
• NCBI Official Synonym Full Names: intraflagellar transport 43
• NCBI Official Symbol: IFT43
• NCBI Official Synonym Symbols: CED3; C14orf179
• NCBI Protein Information: intraflagellar transport protein 43 homolog; IFT complex A subunit; intraflagellar transport 43 homolog
• UniProt Protein Name: Intraflagellar transport protein 43 homolog
• UniProt Gene Name: IFT43
• UniProt Synonym Gene Names: C14orf179
• UniProt Entry Name: IFT43_HUMAN

Uniprot Description

Function: Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base. Ref.7

Subunit structure: Component of the IFT complex A (IFT-A) complex

Probable. Interacts with WDR35/IFT121. Ref.4 Ref.5

Subcellular location: Cytoplasm › cytoskeleton. Note: Associated with microtubules. Ref.8

Involvement in disease: Cranioectodermal dysplasia 3 (CED3) [MIM:614099]: A disorder primarily characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include craniosynostosis, narrow rib cage, short limbs, brachydactyly, hypoplastic and widely spaced teeth, sparse hair, skin laxity and abnormal nails. Nephronophthisis leading to progressive renal failure, hepatic fibrosis, heart defects, and retinitis pigmentosa have also been described.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7

Sequence similarities: Belongs to the IFT43 family.