Rabbit C14orf179 Polyclonal Antibody | anti-IFT43 antibody

C14orf179 Antibody (Center)

Cat. Num. AAA9203073

• Gene Names: IFT43; CED3; C14orf179
• Reactivity: Reactivity: Human, Mouse; Predicted: Bovine
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: C14orf179; Polyclonal Antibody; C14orf179 Antibody (Center); Intraflagellar transport protein 43 homolog; IFT43; anti-IFT43 antibody

• Host: Rabbit
• Reactivity: Reactivity: Human, Mouse; Predicted: Bovine
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This C14orf179 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-171 amino acids from the Central region of human C14orf179.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 145-171

• Applicable Applications for anti-IFT43 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC), ELISA (EIA)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Function: As a component of IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G proteincoupled receptors (GPCRs), it is involved in ciliogenesis (PubMed:28400947, PubMed:28973684). Involved in retrograde ciliary transport along microtubules from the ciliary tip to the base (PubMed:21378380).
• Cellular Location: Cytoplasm, cytoskeleton. Cell projection, cilium Note=Associated with microtubules (PubMed:22361696). Localized at the distal tip of the cilium (PubMed:28973684)
• Tissue Location: Expressed in the retina, predominantly in the photoreceptor outer segment.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(C14orf179 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the C14orf179 antibody detected the C14orf179 protein (arrow).)

Immunohistochemistry (IHC)

(C14orf179 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C14orf179 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(C14orf179 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IFT43 antibody

Signal Transduction

References

Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Heilig, R., et al. Nature 421(6923):601-607(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 156523264
• NCBI GeneID: 112752
• NCBI Accession #: NP_001096034.1; NP_001242924.1; NP_443105.2
• NCBI GenBank Nucleotide #: NM_001102564.1
• UniProt Accession #: Q96FT9; O95418; Q9ULA9; B3KPT6; B4DZI9; G3V385
• Molecular Weight: 23529
• NCBI Official Full Name: intraflagellar transport protein 43 homolog isoform 2
• NCBI Official Synonym Full Names: intraflagellar transport 43
• NCBI Official Symbol: IFT43
• NCBI Official Synonym Symbols: CED3; C14orf179
• NCBI Protein Information: intraflagellar transport protein 43 homolog
• UniProt Protein Name: Intraflagellar transport protein 43 homolog
• Protein Family: Intraflagellar transport protein
• UniProt Gene Name: IFT43
• UniProt Synonym Gene Names: C14orf179
• UniProt Entry Name: IFT43_HUMAN

NCBI Description

This gene encodes a subunit of the intraflagellar transport complex A (IFT-A). IFT-A is a multiprotein complex that plays an important role in cilia assembly and maintenance by mediating retrograde ciliary transport. Mutations in this gene are a cause of cranioectodermal dysplasia-3 (CED3), also known as Sensenbrenner syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

IFT43: Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base. Defects in IFT43 are the cause of cranioectodermal dysplasia type 3 (CED3). A disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include craniosynostosis, narrow rib cage, short limbs, brachydactyly, hypoplastic and widely spaced teeth, sparse hair, skin laxity and abnormal nails. Nephronophthisis leading to progressive renal failure, hepatic fibrosis, heart defects, and retinitis pigmentosa have also been described. Belongs to the IFT43 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: microtubule cytoskeleton; cytoplasm; microtubule organizing center

Biological Process: organelle organization and biogenesis

Disease: Cranioectodermal Dysplasia 3