Rabbit anti-Human, Mouse C10orf46 Polyclonal Antibody | anti-C10orf46 antibody

C10orf46, CT (CACUL1, C10orf46, CAC1, CDK2-associated and cullin domain-containing protein 1, Cdk-associated cullin1) (Biotin)

Cat. Num. AAA6279387

• Gene Names: CACUL1; CAC1; C10orf46
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: C10orf46; Polyclonal Antibody; CT (CACUL1; CAC1; CDK2-associated and cullin domain-containing protein 1; Cdk-associated cullin1) (Biotin); CACUL1; Cdk-associated cullin1; anti-C10orf46 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-C10orf46 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: C10orf46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-365 amino acids from the C-terminal region of human C10orf46.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

((LEFT)Western blot analysis of C10orf46 Antibody (C-term) in A375 cell line lysates (35ug/lane).C10orf46 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of C10orf46 Antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane).C10orf46 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-C10orf46 antibody

Antibodies; Cullin

References

Kong, Y., et al. Cell Cycle 8(21):3544-3553(2009). Deloukas, P., et al. Nature 429(6990):375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 109452597
• NCBI GeneID: 143384
• NCBI Accession #: NP_722517.3
• NCBI GenBank Nucleotide #: NM_153810.4
• UniProt Accession #: Q86Y37; Q5XPL7; Q8IY11; Q8N7S4
• Molecular Weight: 28,293 Da
• NCBI Official Full Name: CDK2-associated and cullin domain-containing protein 1
• NCBI Official Synonym Full Names: CDK2-associated, cullin domain 1
• NCBI Official Symbol: CACUL1
• NCBI Official Synonym Symbols: CAC1; C10orf46
• NCBI Protein Information: CDK2-associated and cullin domain-containing protein 1; Cdk-Associated Cullin1
• UniProt Protein Name: CDK2-associated and cullin domain-containing protein 1
• UniProt Gene Name: CACUL1
• UniProt Synonym Gene Names: C10orf46; CAC1
• UniProt Entry Name: CACL1_HUMAN

Uniprot Description

Function: Cell cycle associated protein capable of promoting cell proliferation through the activation of CDK2 at the G1/S phase transition. Ref.6

Subunit structure: Interacts with CDK2. Ref.6

Tissue specificity: Ubiquitously expressed with highest expression in the mammary gland and large intestine. Highly expressed in cancer tissues and cancer cell lines. During cell cycle progression expression is high in G1/S, low in the middle of S phase, and increases again in S/G2 phase. Ref.6

Sequence similarities: Belongs to the cullin family.

Sequence caution: The sequence AAU89709.1 differs from that shown. Reason: Frameshift at position 296.