Rabbit anti-Human RUVBL1 Polyclonal Antibody | anti-RUVBL1 antibody

RUVBL1 (RuvB-like 1, 49kD TATA Box-binding Protein-interacting Protein, 49kD TBP-interacting Protein, TIP49a, Pontin 52, Nuclear Matrix Protein 238, NMP 238, 54kD Erythrocyte Cytosolic Protein, TIP60-associated Protein 54-alpha, TAP54-alpha, INO80 Complex

Cat. Num. AAA6498340

• Gene Names: RUVBL1; RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: RUVBL1; Polyclonal Antibody; RUVBL1 (RuvB-like 1; 49kD TATA Box-binding Protein-interacting Protein; 49kD TBP-interacting Protein; TIP49a; Pontin 52; Nuclear Matrix Protein 238; NMP 238; 54kD Erythrocyte Cytosolic Protein; TIP60-associated Protein 54-alpha; TAP54-alpha; INO80 Complex; anti-RUVBL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RUVBL1.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RUVBL1 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:100; ELISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human RUVBL1
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RUVBL1 Antibody in MCF-7 cell line lysates (35ug/lane). RUVBL1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with RUVBL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RUVBL1 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of RUVBL1 (arrow) using rabbit polyclonal RUVBL1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RUVBL1 gene.)

Product Categories/Family for anti-RUVBL1 antibody

Antibodies; Enzymes

References

1. Feng, Y., et al., Cancer Res. 63(24):8726-8734 (2003). 2. Hawley, S.B., et al., J. Biol. Chem. 276(1):179-186 (2001). 3. Ikura, T., et al., Cell 102(4):463-473 (2000). 4. Salzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999). 5. Makino, Y., et al., Biochem. Biophys. Res. Commun. 245(3):819-823 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4506753
• NCBI GeneID: 8607
• NCBI Accession #: NP_003698
• NCBI GenBank Nucleotide #: NM_003707.2
• UniProt Accession #: Q9Y265; P82276; Q1KMR0; Q53HK5; Q53HL7; Q53Y27; Q9BSX9; B2R5S0
• Molecular Weight: 42,127 Da
• NCBI Official Full Name: ruvB-like 1 isoform 1
• NCBI Official Synonym Full Names: RuvB like AAA ATPase 1
• NCBI Official Symbol: RUVBL1
• NCBI Official Synonym Symbols: RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52
• NCBI Protein Information: ruvB-like 1
• UniProt Protein Name: RuvB-like 1
• Protein Family: RuvB
• UniProt Gene Name: RUVBL1
• UniProt Synonym Gene Names: INO80H; NMP238; TIP49; TIP49A; 49 kDa TBP-interacting protein; ECP-54; NMP 238; TAP54-alpha
• UniProt Entry Name: RUVB1_HUMAN

NCBI Description

This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

RUVBL1: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex. Belongs to the ruvB family. Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Motility/polarity/chemotaxis; Helicase

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: nucleoplasm; Golgi apparatus; NuA4 histone acetyltransferase complex; nuclear matrix; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus

Molecular Function: protein binding; DNA helicase activity; ATPase activity; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; nucleosome assembly; mitosis; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; cell division; regulation of growth; spermatogenesis; DNA repair; DNA duplex unwinding; DNA recombination