Rabbit c-PLA2 Polyclonal Antibody | anti-c-PLA2 antibody

c-PLA2 (N499) Polyclonal Antibody

Cat. Num. AAA3001068

• Gene Names: PLA2G4A; GURDP; cPLA2; PLA2G4; cPLA2-alpha
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: c-PLA2; Polyclonal Antibody; c-PLA2 (N499) Polyclonal Antibody; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase Lysophospholipase; PLA2G4A; CPLA2; PLA2G4; anti-c-PLA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: c-PLA2 (N499) polyclonal antibody detects endogenous levels of c-PLA2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 749

• Applicable Applications for anti-c-PLA2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 480-530 of Human c-PLA2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of c-PLA2 (N499) pAb at 1:500 dilutionLane1:THP-1 whole cell lysate (40ug) Lane2:MEF whole cell lysate (10ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:K562 whole cell lysate (10ug) Lane5:Jurkat whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of c-PLA2 (N499) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of c-PLA2 (N499) pAb at 1:500 dillutionLane1:A549 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (15ug))

Related Product Information for anti-c-PLA2 antibody

Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts; and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.

NCBI and Uniprot Product Information

• NCBI GI #: 23943920
• NCBI GeneID: 5321
• NCBI Accession #: NP_077734.1
• NCBI GenBank Nucleotide #: NP_077734.1
• UniProt Accession #: P47712
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: cytosolic phospholipase A2 isoform 1
• NCBI Official Synonym Full Names: phospholipase A2 group IVA
• NCBI Official Symbol: PLA2G4A
• NCBI Official Synonym Symbols: GURDP; cPLA2; PLA2G4; cPLA2-alpha
• NCBI Protein Information: cytosolic phospholipase A2
• UniProt Protein Name: Cytosolic phospholipase A2
• UniProt Gene Name: PLA2G4A
• UniProt Synonym Gene Names: CPLA2; PLA2G4; cPLA2
• UniProt Entry Name: PA24A_HUMAN

NCBI Description

This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

cPLA2: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Arachidonic acid is a precursor for the eicosanoids that are involved in hemodynamic regulation, inflammatory responses, and other cellular processes. Promotes cerebellar long-term depression and motor learning. Translocates to the Golgi and endoplasmic reticulum in a calcium-dependent fashion. Translocation and activation of at the ER facilitates the process of ER stress. Regulates the biogenesis of lipid droplets, a process dependent on JNK and ceramide kinase. Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+. The N-terminal C2 domain, by its association with lipid membranes, mediates the regulation of CPLA2 by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Inhibited when in trimolecular complex with ANXA2 and S100A10. Phosphorylation of S727 relieves this inhibitory interaction, thus activating PLA2G4A.

Protein type: Lipid Metabolism - ether lipid; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - arachidonic acid; EC 3.1.1.5; Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasm; cytoplasmic membrane-bound vesicle; mitochondrial inner membrane; cytosol

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; calcium-dependent phospholipid binding; calcium ion binding; lysophospholipase activity

Biological Process: icosanoid biosynthetic process; platelet activating factor biosynthetic process; platelet activation; phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; icosanoid metabolic process; phosphatidic acid biosynthetic process; arachidonic acid metabolic process; arachidonic acid secretion; blood coagulation; regulation of cell proliferation