Rabbit c-Jun Polyclonal Antibody | anti-c-Jun antibody

c-Jun (Phospho-Ser73) Antibody

Cat. Num. AAA9412778

• Gene Names: JUN; AP1; AP-1; c-Jun
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: c-Jun; Polyclonal Antibody; c-Jun (Phospho-Ser73) Antibody; AH119; AP1; Jun A; p39; anti-c-Jun antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 331

• Applicable Applications for anti-c-Jun antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.
• Modification: Phospho-Ser73
• Target Name: c-Jun
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Western Blot (WB)

(Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody.)

Related Product Information for anti-c-Jun antibody

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

Product Categories/Family for anti-c-Jun antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4758616
• NCBI GeneID: 3725
• NCBI Accession #: NP_002219.1
• NCBI GenBank Nucleotide #: NM_002228.3
• UniProt Accession #: P05412; Q6FHM7; Q96G93
• Molecular Weight: 35,676 Da
• NCBI Official Full Name: transcription factor AP-1
• NCBI Official Synonym Full Names: jun proto-oncogene
• NCBI Official Symbol: JUN
• NCBI Official Synonym Symbols: AP1; AP-1; c-Jun
• NCBI Protein Information: transcription factor AP-1
• UniProt Protein Name: Transcription factor AP-1
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: JUN
• UniProt Synonym Gene Names: AP1
• UniProt Entry Name: JUN_HUMAN

NCBI Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).