Rabbit Butyrate Response Factor 1/2 Polyclonal Antibody | anti-ZFP36L1 antibody

Butyrate Response Factor 1/2 (BRF 1/2)

Cat. Num. AAA610750

• Gene Names: ZFP36L1; BRF1; ERF1; cMG1; ERF-1; Berg36; TIS11B; RNF162B
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Butyrate Response Factor 1/2; Polyclonal Antibody; Butyrate Response Factor 1/2 (BRF 1/2); Anti -Butyrate Response Factor 1/2 (BRF 1/2); anti-ZFP36L1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human BRF1 and BRF2 proteins. Species Crossreactivity: mouse, rat and monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-ZFP36L1 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry.; Dilution: Western Blot: 1:1000; Immunofluorescence (IF-IC): 1:25; Flow Cytometry: 1:100
• Immunogen: Synthetic peptide corresponding to the carboxy terminus of human BRF1 protein. MW: 40-50kD; BRF1, 62kD; BRF2
• Species Sequence Homology: Bovine, chicken, 100%
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of cell lysates from HT1080, HT1080 BRF1-/-, HCT15, C6, COS and NIH/3T3 cells, using MBS610750.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of A549 cells, using MBS610750 (blue) compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Immunofluorescent analysis of DLD-1 cells, using MBS610750.)

Related Product Information for anti-ZFP36L1 antibody

Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins. This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3'-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome. These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNF alpha, IL-2, IL-3 and IL-6. Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation. Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1.

Product Categories/Family for anti-ZFP36L1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 15812180
• NCBI GeneID: 677
• NCBI Accession #: NP_004917.2
• NCBI GenBank Nucleotide #: NM_004926.2
• UniProt Accession #: Q07352; Q13851
• Molecular Weight: 36,314 Da
• NCBI Official Full Name: zinc finger protein 36, C3H1 type-like 1
• NCBI Official Synonym Full Names: zinc finger protein 36, C3H type-like 1
• NCBI Official Symbol: ZFP36L1
• NCBI Official Synonym Symbols: BRF1; ERF1; cMG1; ERF-1; Berg36; TIS11B; RNF162B
• NCBI Protein Information: zinc finger protein 36, C3H1 type-like 1; EGF-response factor 1; butyrate response factor 1; early response factor Berg36; zinc finger protein, C3H type, 36-like 1
• UniProt Protein Name: Zinc finger protein 36, C3H1 type-like 1
• Protein Family: mRNA decay activator protein
• UniProt Gene Name: ZFP36L1
• UniProt Synonym Gene Names: BERG36; BRF1; ERF1; RNF162B; TIS11B
• UniProt Entry Name: TISB_HUMAN

NCBI Description

This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. [provided by RefSeq]

Uniprot Description

BRF1: a zinc finger protein that plays an important role in the assembly of the RNA polymerase III initiation factor TFIIIB. Regulates mRNA levels by targeting transcripts containing AREs (AU-rich elements) into the decay pathway. Phosphorylation by Akt apparently generates 14-3-3 binding sites and inhibits BRF1 from promoting mRNA activity.Contains 2 C3H1-type zinc fingers.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 14q22-q24

Cellular Component: nucleus; cytosol

Molecular Function: mRNA binding; protein binding; DNA binding; metal ion binding; transcription factor activity

Biological Process: neural tube development; regulation of translation; cell proliferation; regulation of transcription, DNA-dependent; apoptosis; multicellular organism growth; heart development; T cell differentiation in the thymus; gene expression; regulation of mRNA stability; vasculogenesis; mRNA catabolic process, deadenylation-dependent decay