Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using BUD31 antibody.)
Rabbit BUD31 Polyclonal Antibody | anti-BUD31 antibody
BUD31 Antibody
Gene Names
BUD31; G10; EDG2; Cwc14; EDG-2; fSAP17; YCR063W
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
BUD31; Polyclonal Antibody; BUD31 Antibody; Bud31; BUD31 homolog (S. cerevisiae); BUD31_HUMAN; Cwc14; EDG 2; EDG2; fSAP17; Functional spliceosome associated protein 17; G10; G10 maternal transcript homolog; Maternal G10 transcript; Protein BUD31 homolog; Protein EDG-2; Protein G10 homolog; YCR063W; anti-BUD31 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BUD31 antibody detects endogenous levels of total BUD31
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
144
Applicable Applications for anti-BUD31 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus
Tissue Specificity
Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using BUD31 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using BUD31 antibody.)
Immunofluorescene (IF)
(MBS9604637 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9604637 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-BUD31 antibody
Function: Involved in the pre-mRNA splicing process (PubMed:28502770, PubMed:28076346). May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity (PubMed:25091737).
Subunit Structure: Identified in the spliceosome C complex (PubMed:28502770, PubMed:28076346). May interact with AR (PubMed:25091737).
Similarity: Contains a short sequence motif (Phe-Xaa-Xaa-Phe-Tyr) that can bind to AR and may modulate AR activity. Belongs to the BUD31 (G10) family.
Subunit Structure: Identified in the spliceosome C complex (PubMed:28502770, PubMed:28076346). May interact with AR (PubMed:25091737).
Similarity: Contains a short sequence motif (Phe-Xaa-Xaa-Phe-Tyr) that can bind to AR and may modulate AR activity. Belongs to the BUD31 (G10) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 17 kD
Predicted: 17 kDa
Predicted: 17 kDa
NCBI Official Full Name
protein BUD31 homolog
NCBI Official Synonym Full Names
BUD31 homolog
NCBI Official Symbol
BUD31
NCBI Official Synonym Symbols
G10; EDG2; Cwc14; EDG-2; fSAP17; YCR063W
NCBI Protein Information
protein BUD31 homolog
UniProt Protein Name
Protein BUD31 homolog
Protein Family
UniProt Gene Name
BUD31
UniProt Synonym Gene Names
EDG2
Uniprot Description
Involved in the pre-mRNA splicing process (PubMed:28502770, PubMed:28076346). May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity (PubMed:25091737).
Research Articles on BUD31
Similar Products
Product Notes
The BUD31 bud31 (Catalog #AAA9604637) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BUD31 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BUD31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the BUD31 bud31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUD31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
