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Western Blot (WB)
(BUD13 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)
Rabbit BUD13 Polyclonal Antibody | anti-BUD13 antibody
BUD13 Antibody (C-term)
Gene Names
BUD13; Cwc26; fSAP71
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BUD13; Polyclonal Antibody; BUD13 Antibody (C-term); BUD13 homolog; anti-BUD13 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BUD13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 462-490 amino acids from the C-terminal region of human BUD13.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
462-490
Sequence Length
485
Applicable Applications for anti-BUD13 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(BUD13 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)
Western Blot (WB)
(BUD13 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)
Western Blot (WB)
(BUD13 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)
Western Blot (WB)
(BUD13 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)
Immunohistochemistry (IHC)
(BUD13 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BUD13 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(BUD13 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BUD13 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(BUD13 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(BUD13 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-BUD13 antibody
References
Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010)
Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Suchindran, S., et al. PLoS Genet. 6 (4), E1000928 (2010) :
Hegele, R.A., et al. Hum. Mol. Genet. 18(21):4189-4194(2009)
Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009)
Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010)
Suchindran, S., et al. PLoS Genet. 6 (4), E1000928 (2010) :
Hegele, R.A., et al. Hum. Mol. Genet. 18(21):4189-4194(2009)
Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70521
NCBI Official Full Name
BUD13 homolog isoform 2
NCBI Official Synonym Full Names
BUD13 homolog (S. cerevisiae)
NCBI Official Symbol
BUD13
NCBI Official Synonym Symbols
Cwc26; fSAP71
NCBI Protein Information
BUD13 homolog
UniProt Protein Name
BUD13 homolog
Protein Family
UniProt Gene Name
BUD13
UniProt Entry Name
BUD13_HUMAN
Uniprot Description
BUD13: a conserved protein of unknown function.
Protein type: Spliceosome; RNA splicing
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding
Biological Process: nuclear mRNA splicing, via spliceosome; mRNA export from nucleus
Research Articles on BUD13
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Product Notes
The BUD13 bud13 (Catalog #AAA9211556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 462-490. The BUD13 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's BUD13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the BUD13 bud13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BUD13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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