Rabbit anti-Human, Mouse BUD13 Polyclonal Antibody | anti-BUD13 antibody

BUD13, CT (BUD13, BUD13 homolog) (PE)

Cat. Num. AAA6279340

• Gene Names: BUD13; Cwc26; fSAP71
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BUD13; Polyclonal Antibody; CT (BUD13; BUD13 homolog) (PE); BUD13 homolog; anti-BUD13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 619

• Applicable Applications for anti-BUD13 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BUD13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-494 amino acids from the C-terminal region of human BUD13.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(BUD13 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)

Western Blot (WB)

(BUD13 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the BUD13 antibody detected the BUD13 protein (arrow).)

Immunohistochemistry (IHC)

(BUD13 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BUD13 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(BUD13 Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-BUD13 antibody

Antibodies; Proteins

References

Stein, J.L., et al. Neuroimage 53(3):1160-1174(2010). Fontaine-Bisson, B., et al. Diabetologia 53(10):2155-2162(2010). Suchindran, S., et al. PLoS Genet. 6 (4), E1000928 (2010): Hegele, R.A., et al. Hum. Mol. Genet. 18(21):4189-4194(2009). Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 14249338
• NCBI GeneID: 84811
• NCBI Accession #: NP_116114.1
• NCBI GenBank Nucleotide #: NP_116114.1
• UniProt Accession #: Q9BRD0
• NCBI Official Full Name: BUD13 homolog isoform 1
• NCBI Official Synonym Full Names: BUD13 homolog
• NCBI Official Symbol: BUD13
• NCBI Official Synonym Symbols: Cwc26; fSAP71
• NCBI Protein Information: BUD13 homolog
• UniProt Protein Name: BUD13 homolog
• Protein Family: BUD13
• UniProt Gene Name: BUD13
• UniProt Entry Name: BUD13_HUMAN

Uniprot Description

BUD13: a conserved protein of unknown function.

Protein type: RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; mRNA export from nucleus