Mouse anti-Human HERC3 Monoclonal Antibody | anti-HERC3 antibody

HERC3 (Probable E3 Ubiquitin-protein Ligase HERC3, HECT Domain and RCC1-like Domain-containing Protein 3, KIAA0032) (Biotin)

Cat. Num. AAA6142257

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HERC3; Monoclonal Antibody; HERC3 (Probable E3 Ubiquitin-protein Ligase HERC3; HECT Domain and RCC1-like Domain-containing Protein 3; KIAA0032) (Biotin); anti-HERC3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2C1
• Specificity: Recognizes human HERC3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 1050

• Applicable Applications for anti-HERC3 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-110 from human HERC3 (NP_055421) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWG
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Immunoperoxidase of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged HERC3 is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-HERC3 antibody

Antibodies; Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 7657152
• NCBI GeneID: 8916
• NCBI Accession #: NP_055421
• NCBI GenBank Nucleotide #: NM_014606
• UniProt Accession #: Q15034
• NCBI Official Full Name: probable E3 ubiquitin-protein ligase HERC3 isoform 1
• NCBI Official Synonym Full Names: HECT and RLD domain containing E3 ubiquitin protein ligase 3
• NCBI Official Symbol: HERC3
• NCBI Protein Information: probable E3 ubiquitin-protein ligase HERC3
• UniProt Protein Name: Probable E3 ubiquitin-protein ligase HERC3
• Protein Family: Probable E3 ubiquitin-protein ligase
• UniProt Gene Name: HERC3
• UniProt Synonym Gene Names: KIAA0032

NCBI Description

This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]

Uniprot Description

HERC3: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol

Biological Process: protein ubiquitination