Rabbit anti-Human ARL2 Polyclonal Antibody | anti-ARL2 antibody

ARL2, CT (ARL2, ADP-ribosylation factor-like protein 2) (PE)

Cat. Num. AAA6275137

• Gene Names: ARL2; ARFL2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ARL2; Polyclonal Antibody; CT (ARL2; ADP-ribosylation factor-like protein 2) (PE); ADP-ribosylation factor-like protein 2; anti-ARL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ARL2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: ARL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ARL2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ARL2 (arrow) using rabbit polyclonal ARL2 Antibody (D170). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARL2 gene.)

Immunohistochemistry (IHC)

(ARL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ARL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ARL2 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ARL2 antibody

The ADP-ribosylation factor (ARF) genes are small GTP-binding proteins of the RAS superfamily. ARL2 is a member of a functionally distinct group of ARF-like genes. This protein is a component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine.

Product Categories/Family for anti-ARL2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Antoshechkin, I., et al., Dev. Cell 2(5):579-591 (2002). Bhamidipati, A., et al., J. Cell Biol. 149(5):1087-1096 (2000). Guru, S.C., et al., Genome Res. 7(7):725-735 (1997). Clark, J., et al., Proc. Natl. Acad. Sci. U.S.A. 90(19):8952-8956 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 116241255
• NCBI GeneID: 402
• UniProt Accession #: P36404; Q9BUK8; G3V184
• Molecular Weight: 184
• NCBI Official Full Name: ADP-ribosylation factor-like protein 2
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 2
• NCBI Official Symbol: ARL2
• NCBI Official Synonym Symbols: ARFL2
• NCBI Protein Information: ADP-ribosylation factor-like protein 2
• UniProt Protein Name: ADP-ribosylation factor-like protein 2
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL2
• UniProt Entry Name: ARL2_HUMAN

NCBI Description

This gene encodes a small GTP-binding protein of the RAS superfamily which functions as an ADP-ribosylation factor (ARF). The encoded protein is one of a functionally distinct group of ARF-like genes. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL2: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; Mitochondrial; G protein, monomeric

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: centrosome; mitochondrial intermembrane space; nucleus; lateral plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTPase inhibitor activity

Biological Process: tubulin folding; centrosome organization and biogenesis; metabolic process; small GTPase mediated signal transduction; positive regulation of microtubule polymerization; maintenance of protein localization in nucleus; cell cycle; regulation of microtubule polymerization