Western Blot (WB)
(Western blot analysis of EN2 antibody (C-term) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse EN2 Polyclonal Antibody | anti-EN2 antibody
EN2, CT (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2) (HRP)
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of EN2 antibody (C-term) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of EN2 antibody (C-term) in 293 cell line lysates and mouse stomach and lung tissue lysates (35ug/lane). EN2 (arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly. )
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with EN2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). EN2 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly. )
NCBI and Uniprot Product Information
NCBI Description
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
En-2: Genetic variations in EN2 may be associated with susceptibility to autism. Belongs to the engrailed homeobox family.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: membrane; nucleus
Molecular Function: sequence-specific DNA binding
Biological Process: multicellular organismal development; midbrain development; neuron development; positive regulation of transcription from RNA polymerase II promoter; hindbrain development
Disease: Autism, Susceptibility To, 10
Research Articles on EN2
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Product Notes
The EN2 en2 (Catalog #AAA6473809) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EN2, CT (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EN2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EN2 en2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EN2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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