Rabbit anti-Human BTN3A3 Polyclonal Antibody | anti-BTN3A3 antibody

BTN3A3, NT (BTN3A3, BTF3, Butyrophilin subfamily 3 member A3) (FITC)

Cat. Num. AAA6279278

• Gene Names: BTN3A3; BTF3; BTN3.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BTN3A3; Polyclonal Antibody; NT (BTN3A3; BTF3; Butyrophilin subfamily 3 member A3) (FITC); Butyrophilin subfamily 3 member A3; anti-BTN3A3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 535

• Applicable Applications for anti-BTN3A3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BTN3A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human BTN3A3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BTN3A3 Antibody (N-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the BTN3A3 antibody detected the BTN3A3 protein (arrow).)

Product Categories/Family for anti-BTN3A3 antibody

Antibodies; Proteins

References

Smith, I.A., et al. J. Immunol. 184(7):3514-3525(2010). Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). Rhodes, D.A., et al. Genomics 71(3):351-362(2001). Henry, J., et al. Immunol. Today 20(6):285-288(1999). Ruddy, D.A., et al. Genome Res. 7(5):441-456(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 261490684
• NCBI GeneID: 10384
• NCBI Accession #: NP_932078.2
• NCBI GenBank Nucleotide #: NP_932078.2
• UniProt Accession #: O00478
• NCBI Official Full Name: butyrophilin subfamily 3 member A3 isoform b
• NCBI Official Synonym Full Names: butyrophilin subfamily 3 member A3
• NCBI Official Symbol: BTN3A3
• NCBI Official Synonym Symbols: BTF3; BTN3.3
• NCBI Protein Information: butyrophilin subfamily 3 member A3
• UniProt Protein Name: Butyrophilin subfamily 3 member A3
• Protein Family: Butyrophilin subfamily
• UniProt Gene Name: BTN3A3
• UniProt Synonym Gene Names: BTF3
• UniProt Entry Name: BT3A3_HUMAN

NCBI Description

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Uniprot Description

Function: Plays a role in T-cell responses in the adaptive immune response. Ref.10

Subunit structure: Homodimer. Ref.11

Subcellular location: Cell membrane; Single-pass type I membrane protein Ref.9 Ref.10.

Tissue specificity: Detected in peripheral blood mononuclear cells and in T-cells (at protein level). Detected in spleen and lymphocytes. Ref.8

Post-translational modification: N-glycosylated. Ref.8

Sequence similarities: Belongs to the immunoglobulin superfamily. BTN/MOG family.Contains 1 B30.2/SPRY domain.Contains 2 Ig-like V-type (immunoglobulin-like) domains.