Rabbit BTG1 Polyclonal Antibody | anti-BTG1 antibody

BTG1 Polyclonal Antibody

Cat. Num. AAA3011521

• Gene Names: BTG1; APRO2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BTG1; Polyclonal Antibody; BTG1 Polyclonal Antibody; Protein BTG1; B-cell translocation gene 1 protein; anti-BTG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BTG1 polyclonal antibody detects endogenous levels of BTG1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 171

• Applicable Applications for anti-BTG1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human BTG1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using BTG1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-BTG1 antibody

B cell translocation gene proteins, also designated BTG-1-4, are members of a novel anti-proliferative gene family and play a role in transcription regulation. BTG genes are considered immediate early genes whose expression is induced in response to mitogenic as well as differentiative and antiproliferative factors. Expression of BTG1 is maximal in the G0/G1 phases of the cell cycle and is downregulated when cells progress through G1. BTG2 is a p53 inducible, anti-proliferative protein that regulates the G1/S transition of the cell cycle. BTG2 expression increases in response to DNA damage, cell differentiation, cell quiescence, cell contact and as part of a positive feedback mechanism in response to growth stimulation. High levels of BTG2 are present in kidney proximal tubules, lung alveolar bronchial epithelium, and the basal cell layer of prostate acini. BTG1 and BTG2 both contain LXXLL motifs, referred to as nuclear receptor boxes, which are involved in the regulation of ER-mediated activation. Human BTG3 protein is abundantly expressed in testis, prostate, ovary, thymus and lung.

NCBI and Uniprot Product Information

• NCBI GI #: 4502473
• NCBI GeneID: 694
• NCBI Accession #: NP_001722.1
• NCBI GenBank Nucleotide #: NP_001722.1
• UniProt Accession #: P62324
• Molecular Weight: ~ 19kDa
• NCBI Official Full Name: protein BTG1
• NCBI Official Synonym Full Names: BTG anti-proliferation factor 1
• NCBI Official Symbol: BTG1
• NCBI Official Synonym Symbols: APRO2
• NCBI Protein Information: protein BTG1
• UniProt Protein Name: Protein BTG1
• Protein Family: Protein
• UniProt Gene Name: BTG1
• UniProt Entry Name: BTG1_HUMAN

NCBI Description

This gene is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. [provided by RefSeq, Oct 2008]

Uniprot Description

BTG1: Anti-proliferative protein. A chromosomal aberration involving BTG1 may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with MYC. Belongs to the BTG family.

Protein type: Cell cycle regulation; Oncoprotein

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; transcription cofactor activity; kinase binding

Biological Process: negative regulation of cell proliferation; positive regulation of angiogenesis; cell migration; positive regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; negative regulation of cell growth; positive regulation of myoblast differentiation