Rabbit anti-Human, Mouse BTG1 Polyclonal Antibody | anti-BTG1 antibody

BTG1, ID (BTG1, Protein BTG1, B Cell translocation gene 1 protein) (AP)

Cat. Num. AAA6279187

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BTG1; Polyclonal Antibody; ID (BTG1; Protein BTG1; B Cell translocation gene 1 protein) (AP); B-cell translocation gene 1 protein; anti-BTG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-BTG1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the Central region of human BTG1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

((LEFT)Western blot analysis of BTG1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody (Center) in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(BTG1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BTG1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-BTG1 antibody

BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.

Product Categories/Family for anti-BTG1 antibody

Antibodies; Proteins

References

Camina-Tato, M., et al. J. Neuroimmunol. 213 (1-2), 142-147 (2009). Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009). Takahashi, N., et al. Exp. Hematol. 36(12):1760-1770(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 209447059
• NCBI GeneID: 694
• NCBI Accession #: NP_001722.1
• NCBI GenBank Nucleotide #: NM_001731
• UniProt Accession #: P62324; P31607
• Molecular Weight: 19,209 Da
• NCBI Official Full Name: Homo sapiens B-cell translocation gene 1, anti-proliferative (BTG1), mRNA
• NCBI Official Synonym Full Names: B-cell translocation gene 1, anti-proliferative
• NCBI Official Symbol: BTG1
• NCBI Protein Information: protein BTG1; B-cell translocation gene 1 protein
• UniProt Protein Name: Protein BTG1
• Protein Family: Protein
• UniProt Gene Name: BTG1
• UniProt Entry Name: BTG1_HUMAN

NCBI Description

This gene is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. [provided by RefSeq, Oct 2008]

Uniprot Description

BTG1: Anti-proliferative protein. A chromosomal aberration involving BTG1 may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with MYC. Belongs to the BTG family.

Protein type: Cell cycle regulation; Oncoprotein

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; transcription cofactor activity; kinase binding

Biological Process: negative regulation of cell proliferation; positive regulation of angiogenesis; cell migration; positive regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; negative regulation of cell growth; positive regulation of myoblast differentiation