Rabbit anti-Human BTF3 Polyclonal Antibody | anti-BTF3 antibody

BTF3 Antibody

Cat. Num. AAA9629100

• Gene Names: BTF3; NACB; BTF3a; BTF3b; BETA-NAC
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: BTF3; Polyclonal Antibody; BTF3 Antibody; Basic transcription factor 3; BETA NAC; BTF 3; btf3; BTF3_HUMAN; BTF3a; BTF3b; NACB; Nascent polypeptide associated complex beta polypeptide; RNA polymerase B transcription factor 3; Transcription factor BTF3; anti-BTF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: BTF3 Antibody detects endogenous levels of total BTF3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-BTF3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the NAC-beta family.
• Subunit Structure: Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.
• Function: When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
• Note: The heterodimer with NACA is cytoplasmic.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-BTF3 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 83641885
• NCBI GeneID: 689
• NCBI Accession #: NP_001032726.1
• NCBI GenBank Nucleotide #: NM_001037637.1
• UniProt Accession #: P20290; Q13893; Q76M56; A8K510
• Molecular Weight: 17,699 Da
• NCBI Official Full Name: transcription factor BTF3 isoform A
• NCBI Official Synonym Full Names: basic transcription factor 3
• NCBI Official Symbol: BTF3
• NCBI Official Synonym Symbols: NACB; BTF3a; BTF3b; BETA-NAC
• NCBI Protein Information: transcription factor BTF3; NAC-beta; RNA polymerase B transcription factor 3; nascent polypeptide-associated complex subunit beta; nascent-polypeptide-associated complex beta polypeptide
• UniProt Protein Name: Transcription factor BTF3
• Protein Family: Basic transcription factor
• UniProt Gene Name: BTF3
• UniProt Synonym Gene Names: NACB; NAC-beta
• UniProt Entry Name: BTF3_HUMAN

NCBI Description

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

BTF3: a general transcription factor. BTF3 can form a stable complex with RNA polymerase II. An interaction partner of protein kinase CK2. Required for the initiation of transcription. Expressed at high levels in glioblastoma multiforme. Overexpressed in the microsatellite instability (MIN) phenotypes in colorectal cancer. Up-regulated strongly in the mammary gland during pregnancy, suggesting an involvement in alveolar growth. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; Transcription initiation complex

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: transcription from RNA polymerase II promoter; protein transport; regulation of transcription, DNA-dependent; in utero embryonic development