Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG3 Hinge-PE)
Mouse Anti-Human IgG3 (gamma 3 chain specific) Secondary Antibody
Mouse Anti-Human IgG3 (hinge)-PE
Reactivity
Human
Synonyms
Mouse Anti-Human IgG3 (gamma 3 chain specific); Monoclonal Secondary Antibody; Mouse Anti-Human IgG3 (hinge)-PE; Mouse Anti-Human IgG3 (hinge) - Phycoerythrin-Labeled; Mouse Anti-Human IgG3 (gamma 3 chain specific) secondary antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG1 Kappa
Clone Number
HP6050
Specificity
Human IgG3 Hinge
Form/Format
R - phycoerythrin (R - PE) Conjugate
Concentration
0.1 mg/mL (varies by lot)
Application Notes
ELISA – Quality tested
FLISA – Quality tested
ELISpot – Reported in literature
Flow Cytometry – Reported in literature
Immunohistochemistry-Frozen Sections – Reported in literature
Immunohistochemistry-Paraffin Sections – Reported in literature
Immunoblotting – Reported in literature
Immunoprecipitation – Reported in literature
Multiplex – Reported in literature
Purification – Reported in literature
Surface Plasmon Resonance – Reported in literature
ELISA:
Purified (UNLB) antibody: less than or equal to1 ug/mL
AP conjugate: 1:500 – 1:1,000
HRP conjugate: 1:4,000 – 1:10,000
BIOT conjugate: 1:5,000 – 1:10,000
FLISA:FITC, AF488, and AF555 conjugates: 1:200 – 1:400
PE and AF647 conjugates: less than or equal to 1 ug/mL
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
FLISA – Quality tested
ELISpot – Reported in literature
Flow Cytometry – Reported in literature
Immunohistochemistry-Frozen Sections – Reported in literature
Immunohistochemistry-Paraffin Sections – Reported in literature
Immunoblotting – Reported in literature
Immunoprecipitation – Reported in literature
Multiplex – Reported in literature
Purification – Reported in literature
Surface Plasmon Resonance – Reported in literature
ELISA:
Purified (UNLB) antibody: less than or equal to1 ug/mL
AP conjugate: 1:500 – 1:1,000
HRP conjugate: 1:4,000 – 1:10,000
BIOT conjugate: 1:5,000 – 1:10,000
FLISA:FITC, AF488, and AF555 conjugates: 1:200 – 1:400
PE and AF647 conjugates: less than or equal to 1 ug/mL
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Immunogen
Human IgG3 myeloma protein
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent.
Preparation and Storage
The R-phycoerythrin (PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8°C. Do not
freeze!
Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG3 Hinge-PE)
Testing Data
(FLISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG3 Hinge-PE)
Product Categories/Family for Mouse Anti-Human IgG3 (gamma 3 chain specific) secondary antibody
Similar Products
Product Notes
The Mouse Anti-Human IgG3 (gamma 3 chain specific) (Catalog #AAA674086) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human IgG3 (hinge)-PE reacts with Human and may cross-react with other species as described in the data sheet. ELISA – Quality tested FLISA – Quality tested ELISpot – Reported in literature Flow Cytometry – Reported in literature Immunohistochemistry-Frozen Sections – Reported in literature Immunohistochemistry-Paraffin Sections – Reported in literature Immunoblotting – Reported in literature Immunoprecipitation – Reported in literature Multiplex – Reported in literature Purification – Reported in literature Surface Plasmon Resonance – Reported in literature ELISA: Purified (UNLB) antibody: less than or equal to1 ug/mL AP conjugate: 1:500 – 1:1,000 HRP conjugate: 1:4,000 – 1:10,000 BIOT conjugate: 1:5,000 – 1:10,000 FLISA:FITC, AF488, and AF555 conjugates: 1:200 – 1:400 PE and AF647 conjugates: less than or equal to 1 ug/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the Mouse Anti-Human IgG3 (gamma 3 chain specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse Anti-Human IgG3 (gamma 3 chain specific), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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