Rabbit anti-Human, Mouse BSEP Polyclonal Antibody | anti-ABCB11 antibody

BSEP, CT (Bile Salt Export Pump, ATP-binding Cassette Sub-family B Member 11, ABCB11)

Cat. Num. AAA626504

• Gene Names: ABCB11; BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: BSEP; Polyclonal Antibody; CT (Bile Salt Export Pump; ATP-binding Cassette Sub-family B Member 11; ABCB11); Anti -BSEP; anti-ABCB11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABCB11. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ABCB11 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa1045-1075, from the C-terminal region of human ABCB11.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of mouse liver tissue lysate using MBS626504.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using MBS626504, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of MBS626504 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ABCB11 antibody

ABCB11 is involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. It is expressed predominatly, if not exclusively, in the liver, where it is further localized to the canilicular microvilli and to subcanilicular vesicles fo the hepatocytes. Structurally, ABCB11 is a multifunctional polypeptide with two homologus halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.

Product Categories/Family for anti-ABCB11 antibody

Antibodies; Abs to Multi Drug Resistance (MDR)

NCBI and Uniprot Product Information

• NCBI GI #: 21536378
• NCBI GeneID: 8647
• NCBI Accession #: NP_003733.2
• NCBI GenBank Nucleotide #: NM_003742.2
• UniProt Accession #: O95342; Q53TL2; Q9UNB2
• Molecular Weight: 146,407 Da
• NCBI Official Full Name: bile salt export pump
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• NCBI Official Symbol: ABCB11
• NCBI Official Synonym Symbols: BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• NCBI Protein Information: bile salt export pump; sister p-glycoprotein; ABC member 16, MDR/TAP subfamily; ATP-binding cassette sub-family B member 11; progressive familial intrahepatic cholestasis 2
• UniProt Protein Name: Bile salt export pump
• Protein Family: Bile salt export pump
• UniProt Gene Name: ABCB11
• UniProt Synonym Gene Names: BSEP
• UniProt Entry Name: ABCBB_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Membrane protein, integral; Transporter; Transporter, ABC family; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: intercellular canaliculus; membrane; apical part of cell; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: transporter activity; ATPase activity, coupled to transmembrane movement of substances; canalicular bile acid transmembrane transporter activity; bile acid-exporting ATPase activity; sodium-exporting ATPase activity, phosphorylative mechanism; ATP binding

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; transport; canalicular bile acid transport; transmembrane transport

Disease: Cholestasis, Progressive Familial Intrahepatic, 2; Cholestasis, Benign Recurrent Intrahepatic, 2