Mouse Bile salt export pump ELISA Kit | Abcb11 elisa kit

Mouse Bile salt export pump ELISA Kit

Cat. Num. AAA765579

• Gene Names: ABCB11; BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• Reactivity: Mouse
• Synonyms: Bile salt export pump; Mouse Bile salt export pump ELISA Kit; Abcb11/Bsep/Spgp/ATP-binding cassette sub-family B member 11/Sister of P-glycoprotein; Abcb11 elisa kit

• Reactivity: Mouse
• Sequence Length: 1321

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Abcb11 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Abcb11 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Abcb11 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Abcb11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Abcb11 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21536378
• NCBI GeneID: 8647
• NCBI Accession #: NP_003733.2
• NCBI GenBank Nucleotide #: NM_003742.2
• Molecular Weight: 146,407 Da
• NCBI Official Full Name: bile salt export pump
• NCBI Official Synonym Full Names: ATP binding cassette subfamily B member 11
• NCBI Official Symbol: ABCB11
• NCBI Official Synonym Symbols: BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• NCBI Protein Information: bile salt export pump
• UniProt Protein Name: Bile salt export pump
• Protein Family: Bile salt export pump
• UniProt Gene Name: ABCB11
• UniProt Synonym Gene Names: BSEP
• UniProt Entry Name: ABCBB_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ABC family; Hydrolase

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: apical part of cell; integral to plasma membrane; intercellular canaliculus; membrane; plasma membrane

Molecular Function: ATP binding; bile acid-exporting ATPase activity; canalicular bile acid transmembrane transporter activity; sodium-exporting ATPase activity, phosphorylative mechanism; transporter activity

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; bile acid metabolic process; canalicular bile acid transport; transport

Disease: Cholestasis, Benign Recurrent Intrahepatic, 2; Cholestasis, Progressive Familial Intrahepatic, 2