Bovine Bile salt export pump (ABCB11) ELISA Kit | ABCB11 elisa kit

Bovine Bile salt export pump (ABCB11) ELISA Kit

Cat. Num. AAA7234172

• Gene Names: ABCB11; BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• Reactivity: Bovine
• Synonyms: Bile salt export pump (ABCB11); Bovine Bile salt export pump (ABCB11) ELISA Kit; ABCB11 elisa kit

• Reactivity: Bovine

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCB11 elisa kit

Intended Uses: This ABCA13 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ABCA13. This ELISA kit for research use only!

Principle of the Assay: ABCA13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ABCA13 antibody and an ABCA13-HRP conjugate. The assay sample and buffer are incubated together with ABCA13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCA13 concentration since ABCA13 from samples and ABCA13-HRP conjugate compete for the anti-ABCA13 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCA13 from the sample, fewer sites are left to bind ABCA13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCA13 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21536378
• NCBI GeneID: 8647
• NCBI Accession #: NP_003733.2
• NCBI GenBank Nucleotide #: NM_003742.2
• Molecular Weight: 146,407 Da
• NCBI Official Full Name: bile salt export pump
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 11
• NCBI Official Symbol: ABCB11
• NCBI Official Synonym Symbols: BSEP; PGY4; SPGP; ABC16; BRIC2; PFIC2; PFIC-2
• NCBI Protein Information: bile salt export pump; sister p-glycoprotein; ABC member 16, MDR/TAP subfamily; ATP-binding cassette sub-family B member 11; progressive familial intrahepatic cholestasis 2
• UniProt Protein Name: Bile salt export pump
• Protein Family: Bile salt export pump
• UniProt Gene Name: ABCB11
• UniProt Synonym Gene Names: BSEP
• UniProt Entry Name: ABCBB_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCB11: Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2). PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease. Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2). BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, ABC family; Hydrolase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q24

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; apical part of cell; integral to membrane; plasma membrane

Molecular Function: transporter activity; ATPase activity, coupled to transmembrane movement of substances; canalicular bile acid transmembrane transporter activity; bile acid-exporting ATPase activity; sodium-exporting ATPase activity, phosphorylative mechanism; ATP binding

Biological Process: bile acid and bile salt transport; bile acid biosynthetic process; transport; bile acid metabolic process; canalicular bile acid transport; transmembrane transport

Disease: Cholestasis, Progressive Familial Intrahepatic, 2; Cholestasis, Benign Recurrent Intrahepatic, 2