Rabbit anti-Human BRUCE Polyclonal Antibody | anti-BIRC6 antibody

BRUCE Antibody

Cat. Num. AAA151092

• Gene Names: BIRC6; BRUCE; APOLLON
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: BRUCE Antibody is affinity chromatography purified via peptide column.
• Synonyms: BRUCE; Polyclonal Antibody; BRUCE Antibody; APOLLON; KIAA1289; Baculoviral IAP repeat-containing protein 6; BIR repeat-containing ubiquitin-conjugating enzyme; baculoviral IAP repeat-containing protein 6; anti-BIRC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist.
• Purity/Purification: BRUCE Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 4857

• Applicable Applications for anti-BIRC6 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE.
• Buffer: BRUCE Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BRUCE antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunohistochemistry (IHC)

(Immunohistochemistry of BRUCE in human small intestine tissue with BRUCE antibody at 5 μg/mL.)

Related Product Information for anti-BIRC6 antibody

BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.

NCBI and Uniprot Product Information

• NCBI GI #: 153792694
• NCBI GeneID: 57448
• NCBI Accession #: NP_057336
• NCBI GenBank Nucleotide #: NM_016252.3
• UniProt Accession #: Q9NR09; Q9ULD1
• Molecular Weight: 534 kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 6
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 6
• NCBI Official Symbol: BIRC6
• NCBI Official Synonym Symbols: BRUCE; APOLLON
• NCBI Protein Information: baculoviral IAP repeat-containing protein 6; baculoviral IAP repeat-containing 6; ubiquitin-conjugating BIR domain enzyme apollon; ubiquitin-conjugating BIR-domain enzyme apollon; BIR repeat-containing ubiquitin-conjugating enzyme
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 6
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC6
• UniProt Synonym Gene Names: KIAA1289; BRUCE; APOLLON
• UniProt Entry Name: BIRC6_HUMAN

NCBI Description

This gene encodes a protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. This protein inhibits apoptosis by facilitating the degradation of apoptotic proteins by ubiquitination. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC6: Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. Homodimer. Binds the activated, processed forms of CASP3, CASP6, CASP7 and CASP9. Interacts with RNF41, DIABLO/SMAC, HTRA2, KIF23/MKLP1, USP8/UBPY, BIRC5/survivin, MAP2K1/MEK1, RAB8A/RAB8, RAB11A/RAB11, PLK1, EXOC3/SEC6 and EXOC4/SEC8. Expressed in brain cancer cells. Inhibited by DIABLO/SMAC, HTRA2, CASP3, CASP6, CASP7 and CASP9.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 2p22.3

Cellular Component: spindle pole; membrane; microtubule organizing center; trans-Golgi network; midbody; endosome

Molecular Function: protein binding; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: mitosis; apoptosis; cell division; positive regulation of cell proliferation; protein ubiquitination; regulation of cytokinesis; protein amino acid phosphorylation; regulation of cell proliferation; negative regulation of apoptosis