Western Blot (WB)
(Western Blot analysis of BRPF1 expression in transfected 293T cell line by MBS6003867. Lane 1: BRPF1 transfected lysate (134.2kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human BRPF1 Polyclonal Antibody | anti-brpf1 antibody
BRPF1 (Peregrin, Bromodomain and PHD Finger-containing Protein 1, Protein Br140, BR140)
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Purified by Protein A affinity chromatography.
Synonyms
BRPF1; Polyclonal Antibody; BRPF1 (Peregrin; Bromodomain and PHD Finger-containing Protein 1; Protein Br140; BR140); Anti -BRPF1 (Peregrin; anti-brpf1 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BRPF1.
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
0.5 mg/ml (varies by lot)
Sequence
MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQSRPANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTETPAATPKSGKHKNKEKRKDSNHHHHHNVSASTTPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELDEEVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICNDGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAHVVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLYMKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEDEDEEEDEGKGWSSEKVKKAKAKSRIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQRNCDQVGRDSEDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQIAMEMQLTPFLILLRKTLEQLQEKDTGNIFSEPVPLSEVTELDEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLIVSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEERLVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPSSRGSLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGPPKRPGRPPKNRESQMTPSHGGSPVGPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGFSGGNQPVKKSFLVYRNDCSLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTSGTENEAYSVGTGRGVGHSMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYPALIIDPKMPREGMFHHGVPIPVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGVNQDLDKEKMLEGRKSNIRKSVQIAYHRALQHRSKVQGEQSSETSDSD
Applicable Applications for anti-brpf1 antibody
Western Blot (WB), Immunofluorescence (IF) - Other applications not tested.
Application Notes
Immunofluorescence: 10ug/ml - Optimal dilutions to be determined by the researcher.
Grade
Affinitty Purified
Immunogen
Full length protein corresponding to aa1-1220 of human BRPF1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of BRPF1 expression in transfected 293T cell line by MBS6003867. Lane 1: BRPF1 transfected lysate (134.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of BRPF1 expression in transfected 293T cell line by MBS6003867. Lane 1: BRPF1 transfected lysate (134.2kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of of HeLa cells, using MBS6003867 (10ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence of of HeLa cells, using MBS6003867 (10ug/ml).)
Related Product Information for anti-brpf1 antibody
The protein encoded by this gene is expressed ubiquitously and at the highest level in testes and spermatogonia. The protein is localized within nuclei, and it is very similar in structure to two zinc finger proteins, AF10 and AF17. It is suggested that these proteins form a family of regulatory proteins. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-brpf1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
bromodomain and PHD finger containing, 1
NCBI Official Symbol
brpf1
NCBI Protein Information
bromodomain and PHD finger containing, 1
Protein Family
Similar Products
Product Notes
The brpf1 (Catalog #AAA6003867) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BRPF1 (Peregrin, Bromodomain and PHD Finger-containing Protein 1, Protein Br140, BR140) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRPF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF) - Other applications not tested. Immunofluorescence: 10ug/ml - Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the brpf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGVDFDVKTF CHNLRATKPP YECPVETCRK VYKSYSGIEY HLYHYDHDNP PPPQQTPLRK HKKKGRQSRP ANKQSPSPSE VSQSPGREVM SYAQAQRMVE VDLHGRVHRI SIFDNLDVVS EDEEAPEEAP ENGSNKENTE TPAATPKSGK HKNKEKRKDS NHHHHHNVSA STTPKLPEVV YRELEQDTPD APPRPTSYYR YIEKSAEELD EEVEYDMDEE DYIWLDIMNE RRKTEGVSPI PQEIFEYLMD RLEKESYFES HNKGDPNALV DEDAVCCICN DGECQNSNVI LFCDMCNLAV HQECYGVPYI PEGQWLCRRC LQSPSRAVDC ALCPNKGGAF KQTDDGRWAH VVCALWIPEV CFANTVFLEP IDSIEHIPPA RWKLTCYICK QRGSGACIQC HKANCYTAFH VTCAQQAGLY MKMEPVRETG ANGTSFSVRK TAYCDIHTPP GSARRLPALS HSEGEEDEDE EEDEGKGWSS EKVKKAKAKS RIKMKKARKI LAEKRAAAPV VSVPCIPPHR LSKITNRLTI QRKSQFMQRL HSYWTLKRQS RNGVPLLRRL QTHLQSQRNC DQVGRDSEDK NWALKEQLKS WQRLRHDLER ARLLVELIRK REKLKRETIK VQQIAMEMQL TPFLILLRKT LEQLQEKDTG NIFSEPVPLS EVTELDEVPD YLDHIKKPMD FFTMKQNLEA YRYLNFDDFE EDFNLIVSNC LKYNAKDTIF YRAAVRLREQ GGAVLRQARR QAEKMGIDFE TGMHIPHSLA GDEATHHTED AAEEERLVLL ENQKHLPVEE QLKLLLERLD EVNASKQSVG RSRRAKMIKK EMTALRRKLA HQRETGRDGP ERHGPSSRGS LTPHPAACDK DGQTDSAAEE SSSQETSKGL GPNMSSTPAH EVGRRTSVLF SKKNPKTAGP PKRPGRPPKN RESQMTPSHG GSPVGPPQLP IMSSLRQRKR GRSPRPSSSS DSDSDKSTED PPMDLPANGF SGGNQPVKKS FLVYRNDCSL PRSSSDSESS SSSSSSAASD RTSTTPSKQG RGKPSFSRGT FPEDSSEDTS GTENEAYSVG TGRGVGHSMV RKSLGRGAGW LSEDEDSPLD ALDLVWAKCR GYPSYPALII DPKMPREGMF HHGVPIPVPP LEVLKLGEQM TQEAREHLYL VLFFDNKRTW QWLPRTKLVP LGVNQDLDKE KMLEGRKSNI RKSVQIAYHR ALQHRSKVQG EQSSETSDSD. It is sometimes possible for the material contained within the vial of "BRPF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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