Immunofluorescence (IF)
(MBS9605308 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit BRP44L Polyclonal Antibody | anti-BRP44L antibody
BRP44L Antibody
Gene Names
MPC1; MPYCD; BRP44L; CGI-129; SLC54A1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
BRP44L; Polyclonal Antibody; BRP44L Antibody; Apoptosis regulating basic protein; Brain protein 44 like protein; CGI 129; CGI129; dJ68L15.3; HSPC040; HSPC040 protein; Mitochondrial pyruvate carrier 1; MPC1; MPYCD; PNAS 115; PNAS115; pyruvate carrier; Mitochondrial; 1; anti-BRP44L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
BRP44L antibody detects endogenous levels of total BRP44L
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
109
Applicable Applications for anti-BRP44L antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Mitochondrion (inner membrane)
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605308 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605308 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9605308 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9605308 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using BRP44L antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells using BRP44L antibody.)
Related Product Information for anti-BRP44L antibody
Function: Mediates the uptake of pyruvate into mitochondria.
Subunit Structure: The functional 150 kDa pyruvate import complex is a heteromer of MPC1 and MPC2.
Similarity: Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.
Subunit Structure: The functional 150 kDa pyruvate import complex is a heteromer of MPC1 and MPC2.
Similarity: Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 12 kD
Predicted: 13 kDa
Predicted: 13 kDa
NCBI Official Full Name
mitochondrial pyruvate carrier 1 isoform 1
NCBI Official Synonym Full Names
mitochondrial pyruvate carrier 1
NCBI Official Symbol
MPC1
NCBI Official Synonym Symbols
MPYCD; BRP44L; CGI-129; SLC54A1
NCBI Protein Information
mitochondrial pyruvate carrier 1
UniProt Protein Name
Mitochondrial pyruvate carrier 1
UniProt Gene Name
MPC1
UniProt Synonym Gene Names
BRP44L
NCBI Description
The protein encoded by this gene is part of an MPC1/MPC2 heterodimer that is responsible for transporting pyruvate into mitochondria. The encoded protein is found in the inner mitochondrial membrane. Defects in this gene are a cause of mitochondrial pyruvate carrier deficiency. Several transcript variants, some protein coding and one non-protein coding, have been found for this gene. [provided by RefSeq, Aug 2012]
Uniprot Description
Mediates the uptake of pyruvate into mitochondria.
Research Articles on BRP44L
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Product Notes
The BRP44L mpc1 (Catalog #AAA9605308) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRP44L Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BRP44L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the BRP44L mpc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRP44L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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