Western Blot (WB)
(Western blot analysis of C6orf170 Antibody (N-term) in A375 cell line lysates (35ug/lane). C6orf170 (arrow) was detected using the purified Pab.)
Rabbit anti-Human BROMI Polyclonal Antibody | anti-BROMI antibody
BROMI, NT (Protein Broad-minded, C6orf170, C6orf171) (AP)
Gene Names
TBC1D32; BROMI; C6orf170; C6orf171; bA57L9.1; bA301B7.2; dJ310J6.1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
BROMI; Polyclonal Antibody; NT (Protein Broad-minded; C6orf170; C6orf171) (AP); anti-BROMI antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human C6orf170.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-BROMI antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human C6orf170 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of C6orf170 Antibody (N-term) in A375 cell line lysates (35ug/lane). C6orf170 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of C6orf170 Antibody (N-term) in A375 cell line lysates (35ug/lane). C6orf170 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with C6orf170 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with C6orf170 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(C6orf170 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(C6orf170 Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-BROMI antibody
References
1. Ota T., et.al., Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
144,756 Da
NCBI Official Full Name
protein broad-minded
NCBI Official Synonym Full Names
TBC1 domain family, member 32
NCBI Official Symbol
TBC1D32
NCBI Official Synonym Symbols
BROMI; C6orf170; C6orf171; bA57L9.1; bA301B7.2; dJ310J6.1
NCBI Protein Information
protein broad-minded; broad-minded homolog; bA301B7.2 (novel protein); dJ310J6.1 (novel protein)
UniProt Protein Name
Protein broad-minded
UniProt Gene Name
TBC1D32
UniProt Synonym Gene Names
BROMI; C6orf170; C6orf171
UniProt Entry Name
BROMI_HUMAN
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Product Notes
The BROMI tbc1d32 (Catalog #AAA6466040) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BROMI, NT (Protein Broad-minded, C6orf170, C6orf171) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BROMI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BROMI tbc1d32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BROMI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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