Rabbit BRM Polyclonal Antibody | anti-BRM antibody

BRM Polyclonal Antibody

Cat. Num. AAA3005713

• Gene Names: SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BRM; Polyclonal Antibody; BRM Polyclonal Antibody; Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SNF2alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2; SMARCA2; SNF2A; SNF2L2; anti-BRM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BRM polyclonal antibody detects endogenous levels of BRM protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1590

• Applicable Applications for anti-BRM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 1387-1432 of Human BRM.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BRM polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)

Related Product Information for anti-BRM antibody

The Brahma protein (Brm) is an ATPase subunit of the Drosophila melanogaster Brm complex, which is highly related to the mammalian SWI/SNF chromatin-remodeling complex. Brm is a transcriptional activator of Hox genes and associates with nearly all transcriptionally active chromatin in a pattern that is non-overlapping with that of Polycomb, a repressor of Hox gene transcription. The Brm complex is an essential coactivator for the trithorax group protein Zeste, a DNA-binding activator of homeotic genes. Reduction of Brm function dramatically reduces the association of RNA polymerase II with Drosophila salivary gland chromosomes, suggesting that the chromatin remodeling activity of the Brm complex plays a general role in facilitating transcription by RNA polymerase II. Brm acts as a dominant suppressor of the rough eye phenotype that results from a hypomorphic mutation of Drosophila cyclin E by inhibiting S phase entry by acting downstream of cyclin E protein accumulation. The interaction of the Brm complex with chromatin may be modulated by BAP111, which is highly associated with the Brm complex in Drosophila embryos via an HMG domain. Brm is highly expressed in unfertilized eggs and early embryos.

NCBI and Uniprot Product Information

• NCBI GI #: 574957243
• NCBI GeneID: 6595
• NCBI Accession #: NP_001276325.1
• NCBI GenBank Nucleotide #: NP_001276325.1
• UniProt Accession #: P51531
• Molecular Weight: ~ 210kDa
• NCBI Official Full Name: probable global transcription activator SNF2L2 isoform a
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• NCBI Official Symbol: SMARCA2
• NCBI Official Synonym Symbols: BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
• NCBI Protein Information: probable global transcription activator SNF2L2
• UniProt Protein Name: Probable global transcription activator SNF2L2
• Protein Family: ATP-dependent helicase
• UniProt Gene Name: SMARCA2
• UniProt Synonym Gene Names: BAF190B; hBRM

NCBI Description

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]

Uniprot Description

SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: intermediate filament cytoskeleton; intracellular membrane-bound organelle; nBAF complex; npBAF complex; nuclear chromatin; nucleoplasm; nucleus; SWI/SNF complex

Molecular Function: ATP binding; chromatin binding; DNA-dependent ATPase activity; helicase activity; histone binding; protein binding; transcription coactivator activity; transcription regulatory region DNA binding

Biological Process: chromatin remodeling; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spermatid development; transcription from RNA polymerase II promoter

Disease: Nicolaides-baraitser Syndrome