Rabbit anti-Human BRK Polyclonal Antibody | anti-BRK antibody

BRK (Phospho-Tyr342) Antibody

Cat. Num. AAA9416578

• Gene Names: PTK6; BRK
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: BRK; Polyclonal Antibody; BRK (Phospho-Tyr342) Antibody; PTK6; SIK; Src-related intestinal kinase; Tks; Tyrosine-protein kinase BRK; Breast tumor kinase; Protein-tyrosine kinase 6; Protein-tyrosine kinase BRK; PTK6 protein tyrosine kinase 6; anti-BRK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: BRK (Phospho-Tyr342) Antibody detects endogenous levels of BRK only when phosphorylated at Tyr342
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 134

• Applicable Applications for anti-BRK antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human BRK (Phospho-Tyr342)
• Immunogen Type: Peptide
• Modification: Phospho-Tyr342
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis BRK (Phospho-Tyr342) using heatshockK562 whole cell lysates)

Product Categories/Family for anti-BRK antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 373432692
• NCBI GeneID: 5753
• NCBI Accession #: NP_001243287.1
• NCBI GenBank Nucleotide #: NM_001256358.1
• UniProt Accession #: Q13882; Q58F01; B2RCR3; B4DW46
• Molecular Weight: 14,465 Da
• NCBI Official Full Name: protein-tyrosine kinase 6 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine kinase 6
• NCBI Official Symbol: PTK6
• NCBI Official Synonym Symbols: BRK
• NCBI Protein Information: protein-tyrosine kinase 6
• UniProt Protein Name: Protein-tyrosine kinase 6
• Protein Family: BrkA autotransporter
• UniProt Gene Name: PTK6
• UniProt Synonym Gene Names: BRK
• UniProt Entry Name: PTK6_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

Brk: a tyrosine kinase of the Src family. May function as an intracellular signal transducer in epithelial tissues. Very high level expression in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Selectively expressed in breast tumors and cell lines, and perhaps in colon and prostate cancers. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Overexpression in mammary cells leads to EGF sensitization and results in a partially transformed phenotype. Enhances anchorage-independent growth and responsiveness to EGF. RNAi reduces proliferation in breast cancer cells. Kinase-inactive mutant indicates tumor function may be independent of catalytic function.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; nucleoplasm; nucleus; ruffle

Molecular Function: identical protein binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: cell migration; innate immune response; negative regulation of signal transduction; positive regulation of cell cycle; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein