Typical Testing Data/Standard Curve (for reference only)
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Rat Cyclin-A2 ELISA Kit | CCNA2 elisa kit
Rat Cyclin-A2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: CCNA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCNA2 antibody and an CCNA2-HRP conjugate. The assay sample and buffer are incubated together with CCNA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCNA2 concentration since CCNA2 from samples and CCNA2-HRP conjugate compete for the anti-CCNA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCNA2 from the sample, fewer sites are left to bind CCNA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCNA2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008]
Uniprot Description
CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Protein type: Nuclear receptor co-regulator; Cell cycle regulation
Chromosomal Location of Human Ortholog: 4q27
Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus
Molecular Function: protein binding; protein kinase binding
Biological Process: mitosis; organ regeneration; response to glucagon stimulus; positive regulation of transcription, DNA-dependent; response to estradiol stimulus; positive regulation of fibroblast proliferation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; Ras protein signal transduction; regulation of G2/M transition of mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle
Research Articles on CCNA2
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Product Notes
The Rat CCNA2 ccna2 (Catalog #AAA7211946) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211946 ELISA Kit recognizes Rat CCNA2. It is sometimes possible for the material contained within the vial of "Cyclin-A2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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