Rabbit anti-Human, Mouse BRIP1 Polyclonal Antibody | anti-BRIP1 antibody

BRIP1, NT (BRIP1, BACH1, FANCJ, Fanconi anemia group J protein, ATP-dependent RNA helicase BRIP1, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1) (Azide free) (HRP)

Cat. Num. AAA6278905

• Gene Names: BRIP1; OF; BACH1; FANCJ
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BRIP1; Polyclonal Antibody; NT (BRIP1; BACH1; FANCJ; Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1) (Azide free) (HRP); BRCA1-interacting protein C-terminal helicase 1; anti-BRIP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BRIP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BRIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-167 amino acids from the N-terminal region of human BRIP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BRIP1 Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the BRIP1 antibody detected the BRIP1 protein (arrow).)

Western Blot (WB)

(BRIP1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the BRIP1 antibody detected the BRIP1 protein (arrow).)

Related Product Information for anti-BRIP1 antibody

BRIP1 is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1).

Product Categories/Family for anti-BRIP1 antibody

Antibodies; Fanconi Proteins

References

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Bailey, S.D., et al. Diabetes Care (2010). In press: Solyom, S., et al. Fam. Cancer (2010). In press: Xie, J., et al. Oncogene 29(17):2499-2508(2010). Smith, I.M., et al. ORL J. Otorhinolaryngol. Relat. Spec. 72(1):44-50(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 301897118
• NCBI GeneID: 83990
• NCBI Accession #: NP_114432
• NCBI GenBank Nucleotide #: NM_032043
• UniProt Accession #: Q9BX63; Q3MJE2; Q8NCI5
• Molecular Weight: 112,476 Da
• NCBI Official Full Name: Fanconi anemia group J protein
• NCBI Official Synonym Full Names: BRCA1 interacting protein C-terminal helicase 1
• NCBI Official Symbol: BRIP1
• NCBI Official Synonym Symbols: OF; BACH1; FANCJ
• NCBI Protein Information: Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1; BRCA1/BRCA2-associated helicase 1
• UniProt Protein Name: Fanconi anemia group J protein
• Protein Family: Fanconi anemia group J protein
• UniProt Gene Name: BRIP1
• UniProt Synonym Gene Names: BACH1; FANCJ; Protein FACJ; BRCA1-interacting protein 1
• UniProt Entry Name: FANCJ_HUMAN

Uniprot Description

BRIP1: a DNA-dependent ATPase and DNA helicase required for the maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Binds directly to the BRCT domains of BRCA1. Defects in BRIP1 cause of susceptibility to breast cancer and Fanconi anemia. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Belongs to the DEAD box helicase family, DEAH subfamily. Two alternatively spliced human isoforms have been described.

Protein type: Oncoprotein; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 17q22.2

Cellular Component: nuclear membrane; cytoplasm; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; double-strand break repair; DNA damage checkpoint; DNA duplex unwinding

Disease: Fanconi Anemia, Complementation Group J; Breast Cancer; Tracheoesophageal Fistula With Or Without Esophageal Atresia