Rabbit TH / Tyrosine Hydroxylase Polyclonal Antibody | anti-TH antibody

Rabbit Polyclonal (IgG) to Human TH / Tyrosine Hydroxylase

Cat. Num. AAA243580

• Gene Names: TH; TYH; DYT14; DYT5b
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TH / Tyrosine Hydroxylase; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TH / Tyrosine Hydroxylase; Anti-TH / Tyrosine Hydroxylase Antibody (aa41-90) IHC-plus; TH; DYT14; Dystonia 14; TYH; Tyrosine 3-hydroxylase; Tyrosine hydroxylase; DYT5b; Tyrosine 3-monooxygenase; Human TH; Tyrosine Hydroxylase; anti-TH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Tyrosine Hydroxylase (Ab-40) Antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 497

• Applicable Applications for anti-TH antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human Tyrosine Hydroxylase (aa41-90).
• Immunogen: TH / Tyrosine Hydroxylase antibody was raised against synthetic peptide from human Tyrosine Hydroxylase (aa41-90).
• Antigen Modification: aa41-90
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TH / Tyrosine Hydroxylase antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of HUVEC cells, using Tyrosine Hydroxylase (Ab-40) Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from NIH-3T3 cells, treated with Forskolin 40 nM 30', using Tyrosine Hydroxylase (Ab-40) Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-TH antibody

Tyrosine hydroxylase in involved in the conversion of phenylalanine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines and as such has a key role in the physiology of adrenergic neurons. TH is encoded by four distinct mRNAs produced by alternative splicing of a single primary transcript. Expression of the mRNAs varies in different parts of the nervous system. Defects in TH are the cause of autosomal recessive Segawa syndrome.

Product Categories/Family for anti-TH antibody

Family: Monooxygenase

NCBI and Uniprot Product Information

• NCBI GI #: 88900503
• NCBI GeneID: 7054
• NCBI Accession #: NP_000351.2
• NCBI GenBank Nucleotide #: NM_000360.3
• UniProt Accession #: P07101; Q0PWM2; Q0PWM3; Q15585; Q15588; Q15589; Q2M3B4; B7ZL70; B7ZL73
• Molecular Weight: 44,898 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase isoform b
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: TH
• NCBI Official Synonym Symbols: TYH; DYT14; DYT5b
• NCBI Protein Information: tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: TH
• UniProt Synonym Gene Names: TYH; TH
• UniProt Entry Name: TY3H_HUMAN

NCBI Description

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: EC 1.14.16.2; Mitochondrial; Oxidoreductase; Vesicle; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: synaptic vesicle; neuron projection; internal side of plasma membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; cytoplasm; melanosome membrane; terminal button; perikaryon; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: amino acid binding; protein domain specific binding; protein binding; enzyme binding; ferric iron binding; ferrous iron binding; dopamine binding; oxygen binding; tyrosine 3-monooxygenase activity

Biological Process: heart morphogenesis; heart development; response to lipopolysaccharide; dopamine biosynthetic process; phytoalexin metabolic process; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; catecholamine biosynthetic process; response to electrical stimulus; epinephrine biosynthetic process; neurotransmitter biosynthetic process; response to pyrethroid; response to corticosterone stimulus; response to light stimulus; anatomical structure morphogenesis; phthalate metabolic process; mating behavior; social behavior; eye photoreceptor cell development; organ morphogenesis; response to ethanol; circadian sleep/wake cycle; response to zinc ion; response to activity; cerebral cortex development; response to water deprivation; response to peptide hormone stimulus; synaptic transmission, dopaminergic; locomotory behavior; fatty acid metabolic process; response to estradiol stimulus; response to salt stress; regulation of heart contraction; sensory perception of sound; visual perception; glycoside metabolic process; response to nutrient levels; terpene metabolic process; sphingolipid metabolic process; eating behavior; response to amphetamine; multicellular organismal aging; isoquinoline alkaloid metabolic process; response to herbicide; learning; response to ether; synaptic vesicle amine transport; memory; pigmentation; response to hypoxia; embryonic camera-type eye morphogenesis

Disease: Segawa Syndrome, Autosomal Recessive