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Western Blot (WB) (Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue.)

Rabbit anti-Mouse Brevican Polyclonal Antibody | anti-BCAN antibody

Polyclonal Antibody to Brevican (BCAN)

Gene Names
Bcan; Cspg7
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Brevican; Polyclonal Antibody; Polyclonal Antibody to Brevican (BCAN); anti-BCAN antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BCAN. It has beenselected for its ability to recognize BCAN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-BCAN antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
BCAN (Asp23~Arg353)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse, Rat
Conjugate
No Conjugate
Immunogen
Recombinant BCAN (Asp23~Arg353) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2063799
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue.)

Western Blot (WB) (Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Mouse Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant BCAN, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant BCAN, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Brain Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Brain Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Heart Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Heart Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,815 Da
NCBI Official Full Name
brevican core protein isoform 2
NCBI Official Synonym Full Names
brevican
NCBI Official Symbol
Bcan
NCBI Official Synonym Symbols
Cspg7
NCBI Protein Information
brevican core protein
UniProt Protein Name
Brevican core protein
Protein Family
UniProt Gene Name
Bcan

Uniprot Description

BCAN: a chondroitin sulfate proteoglycan expressed in the developing and adult brain, and increases as the brain develops. One of the most abundant chondroitin sulfate proteoglycans in the adult brain. Contains a hyaluronic acid-binding domain in its N-terminus and a lectin-like domain in its C-terminus. A member of the aggrecan/versican family of proteoglycans. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is secreted into extracellular space and associates with the extracellular matrix. Isoform 2 is a GPI-anchored membrane protein.

Protein type: Membrane protein, GPI anchor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 F1|3 38.78 cM

Cellular Component: dendrite; extracellular region; extracellular space; proteinaceous extracellular matrix; synapse

Molecular Function: calcium ion binding; carbohydrate binding; extracellular matrix structural constituent; hyaluronic acid binding

Biological Process: cell adhesion; hippocampus development; skeletal system development

Research Articles on BCAN

Similar Products

Product Notes

The BCAN bcan (Catalog #AAA2027350) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Brevican (BCAN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Brevican can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the BCAN bcan for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Brevican, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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