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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Breast And Kidney Expressed Chemokine Polyclonal Antibody | anti-BRAK antibody

Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK)

Gene Names
Cxcl14; KS1; Kec; BMAC; BRAK; NJAC; MIP-2g; Scyb14; AI414372; bolekine; MIP2gamma; 1110031L23Rik; 1200006I23Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Breast And Kidney Expressed Chemokine; Polyclonal Antibody; Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK); anti-BRAK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BRAK. It has beenselected for its ability to recognize BRAK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SKCKCSRK GPKIRYSDVK KLEMKPKYPH CEEKMVIVTT KSMSRYRGQE HCLHPKLQST KRFIKWYNAW NEKRRVYEE
Sequence Length
99
Applicable Applications for anti-BRAK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
BRAK (Ser23~Glu99)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant BRAK (Ser23~Glu99) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2057034
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Brain tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Brain tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,716 Da
NCBI Official Full Name
C-X-C motif chemokine 14
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 14
NCBI Official Symbol
Cxcl14
NCBI Official Synonym Symbols
KS1; Kec; BMAC; BRAK; NJAC; MIP-2g; Scyb14; AI414372; bolekine; MIP2gamma; 1110031L23Rik; 1200006I23Rik
NCBI Protein Information
C-X-C motif chemokine 14
UniProt Protein Name
C-X-C motif chemokine 14
UniProt Gene Name
Cxcl14
UniProt Synonym Gene Names
Bmac; Kec; Ks1; Mip2g; Scyb14

Uniprot Description

CXCL14: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13|13 B1

Cellular Component: extracellular region; extracellular space; Golgi apparatus

Molecular Function: chemokine activity; cytokine activity

Biological Process: cell-cell signaling; immune response; inner ear development; negative regulation of myoblast differentiation

Research Articles on BRAK

Similar Products

Product Notes

The BRAK cxcl14 (Catalog #AAA2026851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Breast And Kidney Expressed Chemokine (BRAK) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Breast And Kidney Expressed Chemokine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the BRAK cxcl14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SKCKCSRK GPKIRYSDVK KLEMKPKYPH CEEKMVIVTT KSMSRYRGQE HCLHPKLQST KRFIKWYNAW NEKRRVYEE. It is sometimes possible for the material contained within the vial of "Breast And Kidney Expressed Chemokine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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